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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- LF-MA0173 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Rb Monoclonal Antibody (32C8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- A suggested positive control for this product is U-2OS cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 32C8
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Rb1 was performed by loading whole cell lysate in 1X LDS sample buffer with 2-ME from 5 x 10^5 mouse transformed pre-B cells. Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # NP0336BOX). Proteins were transferred to nitrocellulose membrane with wet/tank transfer. Membrane was blocked in 5% milk/TBST. The membrane was probe with a monoclonal anti-Rb1 antibody (Product #LF-MA0173) at a dilution of 1:1000 in 5% milk/TBST at 4oC overnight, followed by a secondary antibody HRP-anti-mouse at a dilution of 1:10000 at room temperature for 1 hour. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # PI-32209). Data courtesy of Thermo Scientific KOL Program.
