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- Antibody Data
- Antigen structure
- References [32]
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- Product number
- MA1-34070 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Rb Monoclonal Antibody (1F8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA1-34070 detects RB1 from mouse, rat, and human samples. MA1-34070 has been successfully used in immunoprecipitation, immunohistochemistry (paraffin tissue), and Western blot applications. A suggested positive control is K562 WCL. The MA1-34070 immunogen is recombinant full length protein (Human).
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1F8
- Vial size
- 500 µL
- Concentration
- 0.1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references High-Risk HPV, Biomarkers, and Outcome in Matched Cohorts of Head and Neck Cancer Patients Positive and Negative for HIV.
CDK 4/6 inhibitor palbociclib (PD0332991) in Rb+ advanced breast cancer: phase II activity, safety, and predictive biomarker assessment.
Immunohistochemical analysis of expressions of RB1, CDK4, HSP90, cPLA2G4A, and CHMP2B is helpful in distinction between myxofibrosarcoma and myxoid liposarcoma.
RB1 gene in Merkel cell carcinoma: hypermethylation in all tumors and concurrent heterozygous deletions in the polyomavirus-negative subgroup.
Human papilloma virus genome is rare in North American non-small cell lung carcinoma patients.
Alteration of G1/S transition regulators influences recurrences in head and neck squamous carcinomas.
Selective inhibition of rRNA transcription downregulates E2F-1: a new p53-independent mechanism linking cell growth to cell proliferation.
HFE polymorphisms influence the response to chemotherapeutic agents via induction of p16INK4A.
Prognostic significance of the alterations of the G1-S checkpoint in localized leiomyosarcoma of the peripheral soft tissue.
Simultaneous aberrations of single CDKN2A network components and a high Rb phosphorylation status can differentiate subgroups of primary cutaneous B-cell lymphomas.
SKI promotes Smad3 linker phosphorylations associated with the tumor-promoting trait of TGFbeta.
Activation of Wnt signaling pathway by human papillomavirus E6 and E7 oncogenes in HPV16-positive oropharyngeal squamous carcinoma cells.
Establishment of ponasterone A-inducible the wild-type p53 protein-expressing clones from HSC-1 cells, cell growth suppression by p53 expression and the suppression mechanism.
E2F4 and ribonucleotide reductase mediate S-phase arrest in colon cancer cells treated with chlorophyllin.
E6 and e7 gene silencing and transformed phenotype of human papillomavirus 16-positive oropharyngeal cancer cells.
Defining a molecular phenotype for benign and malignant parathyroid tumors.
The inhibition of T-cells proliferation by mouse mesenchymal stem cells through the induction of p16INK4A-cyclin D1/cdk4 and p21waf1, p27kip1-cyclin E/cdk2 pathways.
The expressions of the Rb pathway in cervical intraepithelial neoplasia; predictive and prognostic significance.
Epigenetic inactivation of the chromosomal stability control genes BRCA1, BRCA2, and XRCC5 in non-small cell lung cancer.
Dynamic assembly of chromatin complexes during cellular senescence: implications for the growth arrest of human melanocytic nevi.
Different effects of ribosome biogenesis inhibition on cell proliferation in retinoblastoma protein- and p53-deficient and proficient human osteosarcoma cell lines.
Molecular and immunohistochemical analysis of the prognostic value of cell-cycle regulators in urothelial neoplasms of the bladder.
Inhibitory effect of caffeic acid phenethyl ester on the growth of C6 glioma cells in vitro and in vivo.
Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells.
p53, p16 and cyclin D1: molecular determinants of radiotherapy treatment response in oral carcinoma.
The prevalence and clinicopathologic correlate of p16INK4a, retinoblastoma and p53 immunoreactivity in locally advanced urinary bladder cancer.
Evidence that high telomerase activity may induce a senescent-like growth arrest in human fibroblasts.
Flavopiridol increases sensitization to gemcitabine in human gastrointestinal cancer cell lines and correlates with down-regulation of ribonucleotide reductase M2 subunit.
Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes.
Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes.
p14ARF silencing by promoter hypermethylation mediates abnormal intracellular localization of MDM2.
Loss of p16INK4 expression by methylation is associated with lifespan extension of human mammary epithelial cells.
Walline HM, Carey TE, Goudsmit CM, Bellile EL, D'Souza G, Peterson LA, McHugh JB, Pai SI, Lee JJ, Shin DM, Ferris RL, HNC SPORE HIV supplement consortium.
Molecular cancer research : MCR 2017 Feb;15(2):179-188
Molecular cancer research : MCR 2017 Feb;15(2):179-188
CDK 4/6 inhibitor palbociclib (PD0332991) in Rb+ advanced breast cancer: phase II activity, safety, and predictive biomarker assessment.
DeMichele A, Clark AS, Tan KS, Heitjan DF, Gramlich K, Gallagher M, Lal P, Feldman M, Zhang P, Colameco C, Lewis D, Langer M, Goodman N, Domchek S, Gogineni K, Rosen M, Fox K, O'Dwyer P
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Mar 1;21(5):995-1001
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Mar 1;21(5):995-1001
Immunohistochemical analysis of expressions of RB1, CDK4, HSP90, cPLA2G4A, and CHMP2B is helpful in distinction between myxofibrosarcoma and myxoid liposarcoma.
Wang T, Goodman MA, McGough RL, Weiss KR, Rao UN
International journal of surgical pathology 2014 Oct;22(7):589-99
International journal of surgical pathology 2014 Oct;22(7):589-99
RB1 gene in Merkel cell carcinoma: hypermethylation in all tumors and concurrent heterozygous deletions in the polyomavirus-negative subgroup.
Sahi H, Savola S, Sihto H, Koljonen V, Bohling T, Knuutila S
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2014 Dec;122(12):1157-66
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2014 Dec;122(12):1157-66
Human papilloma virus genome is rare in North American non-small cell lung carcinoma patients.
Yanagawa N, Wang A, Kohler D, Santos Gda C, Sykes J, Xu J, Pintilie M, Tsao MS
Lung cancer (Amsterdam, Netherlands) 2013 Mar;79(3):215-20
Lung cancer (Amsterdam, Netherlands) 2013 Mar;79(3):215-20
Alteration of G1/S transition regulators influences recurrences in head and neck squamous carcinomas.
Canzonieri V, Barzan L, Franchin G, Vaccher E, Talamini R, Sulfaro S, Baldassarre G
Journal of cellular physiology 2012 Jan;227(1):233-8
Journal of cellular physiology 2012 Jan;227(1):233-8
Selective inhibition of rRNA transcription downregulates E2F-1: a new p53-independent mechanism linking cell growth to cell proliferation.
Donati G, Brighenti E, Vici M, Mazzini G, Treré D, Montanaro L, Derenzini M
Journal of cell science 2011 Sep 1;124(Pt 17):3017-28
Journal of cell science 2011 Sep 1;124(Pt 17):3017-28
HFE polymorphisms influence the response to chemotherapeutic agents via induction of p16INK4A.
Lee SY, Liu S, Mitchell RM, Slagle-Webb B, Hong YS, Sheehan JM, Connor JR
International journal of cancer 2011 Nov 1;129(9):2104-14
International journal of cancer 2011 Nov 1;129(9):2104-14
Prognostic significance of the alterations of the G1-S checkpoint in localized leiomyosarcoma of the peripheral soft tissue.
Panelos J, Beltrami G, Scoccianti G, Capanna R, Paglierani M, Pepi M, Massi D, Franchi A
Annals of surgical oncology 2011 Feb;18(2):566-71
Annals of surgical oncology 2011 Feb;18(2):566-71
Simultaneous aberrations of single CDKN2A network components and a high Rb phosphorylation status can differentiate subgroups of primary cutaneous B-cell lymphomas.
Kaune KM, Neumann C, Hallermann C, Haller F, Schön MP, Middel P
Experimental dermatology 2011 Apr;20(4):331-5
Experimental dermatology 2011 Apr;20(4):331-5
SKI promotes Smad3 linker phosphorylations associated with the tumor-promoting trait of TGFbeta.
Lin Q, Chen D, Timchenko NA, Medrano EE
Cell cycle (Georgetown, Tex.) 2010 May;9(9):1684-9
Cell cycle (Georgetown, Tex.) 2010 May;9(9):1684-9
Activation of Wnt signaling pathway by human papillomavirus E6 and E7 oncogenes in HPV16-positive oropharyngeal squamous carcinoma cells.
Rampias T, Boutati E, Pectasides E, Sasaki C, Kountourakis P, Weinberger P, Psyrri A
Molecular cancer research : MCR 2010 Mar;8(3):433-43
Molecular cancer research : MCR 2010 Mar;8(3):433-43
Establishment of ponasterone A-inducible the wild-type p53 protein-expressing clones from HSC-1 cells, cell growth suppression by p53 expression and the suppression mechanism.
Hori M, Suzuki K, Udono MU, Yamauchi M, Mine M, Watanabe M, Kondo S, Hozumi Y
Archives of dermatological research 2009 Oct;301(9):631-46
Archives of dermatological research 2009 Oct;301(9):631-46
E2F4 and ribonucleotide reductase mediate S-phase arrest in colon cancer cells treated with chlorophyllin.
Chimploy K, Díaz GD, Li Q, Carter O, Dashwood WM, Mathews CK, Williams DE, Bailey GS, Dashwood RH
International journal of cancer 2009 Nov 1;125(9):2086-94
International journal of cancer 2009 Nov 1;125(9):2086-94
E6 and e7 gene silencing and transformed phenotype of human papillomavirus 16-positive oropharyngeal cancer cells.
Rampias T, Sasaki C, Weinberger P, Psyrri A
Journal of the National Cancer Institute 2009 Mar 18;101(6):412-23
Journal of the National Cancer Institute 2009 Mar 18;101(6):412-23
Defining a molecular phenotype for benign and malignant parathyroid tumors.
Fernandez-Ranvier GG, Khanafshar E, Tacha D, Wong M, Kebebew E, Duh QY, Clark OH
Cancer 2009 Jan 15;115(2):334-44
Cancer 2009 Jan 15;115(2):334-44
The inhibition of T-cells proliferation by mouse mesenchymal stem cells through the induction of p16INK4A-cyclin D1/cdk4 and p21waf1, p27kip1-cyclin E/cdk2 pathways.
Kim JA, Hong S, Lee B, Hong JW, Kwak JY, Cho S, Kim CC
Cellular immunology 2007 Jan;245(1):16-23
Cellular immunology 2007 Jan;245(1):16-23
The expressions of the Rb pathway in cervical intraepithelial neoplasia; predictive and prognostic significance.
Nam EJ, Kim JW, Kim SW, Kim YT, Kim JH, Yoon BS, Cho NH, Kim S
Gynecologic oncology 2007 Jan;104(1):207-11
Gynecologic oncology 2007 Jan;104(1):207-11
Epigenetic inactivation of the chromosomal stability control genes BRCA1, BRCA2, and XRCC5 in non-small cell lung cancer.
Lee MN, Tseng RC, Hsu HS, Chen JY, Tzao C, Ho WL, Wang YC
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Feb 1;13(3):832-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Feb 1;13(3):832-8
Dynamic assembly of chromatin complexes during cellular senescence: implications for the growth arrest of human melanocytic nevi.
Bandyopadhyay D, Curry JL, Lin Q, Richards HW, Chen D, Hornsby PJ, Timchenko NA, Medrano EE
Aging cell 2007 Aug;6(4):577-91
Aging cell 2007 Aug;6(4):577-91
Different effects of ribosome biogenesis inhibition on cell proliferation in retinoblastoma protein- and p53-deficient and proficient human osteosarcoma cell lines.
Montanaro L, Mazzini G, Barbieri S, Vici M, Nardi-Pantoli A, Govoni M, Donati G, Treré D, Derenzini M
Cell proliferation 2007 Aug;40(4):532-49
Cell proliferation 2007 Aug;40(4):532-49
Molecular and immunohistochemical analysis of the prognostic value of cell-cycle regulators in urothelial neoplasms of the bladder.
Yurakh AO, Ramos D, Calabuig-Fariñas S, López-Guerrero JA, Rubio J, Solsona E, Romanenko AM, Vozianov AF, Pellin A, Llombart-Bosch A
European urology 2006 Sep;50(3):506-15; discussion 515
European urology 2006 Sep;50(3):506-15; discussion 515
Inhibitory effect of caffeic acid phenethyl ester on the growth of C6 glioma cells in vitro and in vivo.
Kuo HC, Kuo WH, Lee YJ, Lin WL, Chou FP, Tseng TH
Cancer letters 2006 Mar 28;234(2):199-208
Cancer letters 2006 Mar 28;234(2):199-208
Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells.
Choi JK, Murillo G, Su BN, Pezzuto JM, Kinghorn AD, Mehta RG
The FEBS journal 2006 Dec;273(24):5714-23
The FEBS journal 2006 Dec;273(24):5714-23
p53, p16 and cyclin D1: molecular determinants of radiotherapy treatment response in oral carcinoma.
Jayasurya R, Francis G, Kannan S, Lekshminarayanan K, Nalinakumari KR, Abraham T, Abraham EK, Nair MK
International journal of cancer 2004 May 1;109(5):710-6
International journal of cancer 2004 May 1;109(5):710-6
The prevalence and clinicopathologic correlate of p16INK4a, retinoblastoma and p53 immunoreactivity in locally advanced urinary bladder cancer.
Tzai TS, Tsai YS, Chow NH
Urologic oncology 2004 Mar-Apr;22(2):112-8
Urologic oncology 2004 Mar-Apr;22(2):112-8
Evidence that high telomerase activity may induce a senescent-like growth arrest in human fibroblasts.
Gorbunova V, Seluanov A, Pereira-Smith OM
The Journal of biological chemistry 2003 Feb 28;278(9):7692-8
The Journal of biological chemistry 2003 Feb 28;278(9):7692-8
Flavopiridol increases sensitization to gemcitabine in human gastrointestinal cancer cell lines and correlates with down-regulation of ribonucleotide reductase M2 subunit.
Jung CP, Motwani MV, Schwartz GK
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Aug;7(8):2527-36
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Aug;7(8):2527-36
Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes.
Hennigan RF, Stambrook PJ
Molecular biology of the cell 2001 Aug;12(8):2352-63
Molecular biology of the cell 2001 Aug;12(8):2352-63
Dominant negative c-jun inhibits activation of the cyclin D1 and cyclin E kinase complexes.
Hennigan RF, Stambrook PJ
Molecular biology of the cell 2001 Aug;12(8):2352-63
Molecular biology of the cell 2001 Aug;12(8):2352-63
p14ARF silencing by promoter hypermethylation mediates abnormal intracellular localization of MDM2.
Esteller M, Cordon-Cardo C, Corn PG, Meltzer SJ, Pohar KS, Watkins DN, Capella G, Peinado MA, Matias-Guiu X, Prat J, Baylin SB, Herman JG
Cancer research 2001 Apr 1;61(7):2816-21
Cancer research 2001 Apr 1;61(7):2816-21
Loss of p16INK4 expression by methylation is associated with lifespan extension of human mammary epithelial cells.
Huschtscha LI, Noble JR, Neumann AA, Moy EL, Barry P, Melki JR, Clark SJ, Reddel RR
Cancer research 1998 Aug 15;58(16):3508-12
Cancer research 1998 Aug 15;58(16):3508-12
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of MCF7 (Lane 1), Hep G2 (Lane 2), HT-29 (Lane 3) and K-562 (Lane 4). The blot was probed with Anti- Rb Monoclonal Antibody (Product # MA1-34070, 1:500 dilution) and detected by chemiluminescence using Goat anti Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 106 kDa band corresponding to Rb was detected across the cell lines tested.
