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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 701290 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Rb (Thr821) Recombinant Rabbit Monoclonal Antibody (16H2L1)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.
- Antibody clone number
- 16H2L1
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Rb pThr821 in whole cell extracts from COLO 205 cells using a Phospho-Rb pThr821 recombinant rabbit monoclonal antibody (Product # 701290) at a dilution of 2 µg/mL. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lane 2). Results show a band at ~110kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-Rb pThr821 in HeLa cells using a Phospho-Rb pThr821 recombinant rabbit monoclonal antibody (Product # 701290) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of Rb (pT821) and Image E is a composite image of cells showing inhibition of antibody binding after competition with the phosphorylated phosopeptide.
