Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-B77 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B77, RRID:AB_2195446
- Product name
- IHC-plus™ Sprouty 4 / SPRY4 Antibody (aa275-299) LS-B77
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-Sprouty-4 Antibody - Western Blot. Western blot of Affinity Purified anti-Sprouty-4 antibody shows detection of a doublet band ~35 kD corresponding to human Sprouty-4. Approximately 30 ug of HeLa (lane 1), SW13 (lane 2) and C2C12 (Lane 3) whole cell lysates were separated by 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% non-fat dry milk in TBST the membrane was probed with the primary antibody diluted to 1:100 overnight at 4C followed by washes and reaction with a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (LS-C60865) for 1 h at room temperature. Signal was procebetaed by ECL (Pierce). Other detection systems will yield similar results.