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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- TA319359 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Sprouty-4 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Sprouty-4 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SPRY4
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.1 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-Sprouty-4 antibody. Lane 1: HeLa. Lane 2: SW13. Lane 3: C2C12. Load: 30 ug per lane. Primary antibody: Sprouty 4 antibody at 1:100 for overnight at 4°C. Secondary antibody: IRDye800? rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: a doublet band ~35 kDa corresponding to human Sprouty-4. Other band(s): none.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human heart tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Sprouty-4 antibody at 2.5 ug/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Sprouty-4 is cytoplasmic. Staining: Sprouty-4 as precipitated red signal with hematoxylin purple nuclear counterstain.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Sprouty-4 antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Sprouty-4 is cytoplasmic. Staining: Sprouty-4 as precipitated brown with blue nuclear counterstain.
- Validation comment
- IHC
