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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-31401 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPRY4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, mouse brain.
- Concentration
- 1 mg/mL
Submitted references The Role of the miR-21/SPRY2 Axis in Modulating Proangiogenic Factors, Epithelial Phenotypes, and Wound Healing in Corneal Epithelial Cells.
Zhang Y, Yuan F, Liu L, Chen Z, Ma X, Lin Z, Zou J
Investigative ophthalmology & visual science 2019 Sep 3;60(12):3854-3862
Investigative ophthalmology & visual science 2019 Sep 3;60(12):3854-3862
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SPRY4 Polyclonal Antibody (Product # PA5-31401). Sample (30 µg of whole cell lysate). Lane A: NT2D1. 12% SDS PAGE. SPRY4 Polyclonal Antibody (Product # PA5-31401) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SPRY4 Polyclonal Antibody (Product # PA5-31401). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. SPRY4 Polyclonal Antibody (Product # PA5-31401) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SPRY4 Polyclonal Antibody detects SPRY4 protein at cytoplasm on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. SPRY4 Polyclonal Antibody (Product # PA5-31401) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SPRY4 Polyclonal Antibody detects SPRY4 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. SPRY4 Polyclonal Antibody (Product # PA5-31401) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 SPRY2 is a direct target of miR-21 in corneal epithelial cells. Corneal epithelial cells were treated with miR-21 inhibitor and exposed to TGF-beta1 (T), 1% O 2 (H), or TGF-beta1 plus 1% O 2 (T+H). The protein levels of SPRY1, SPRY2, SPRY4, and p-ERK, were determined by Western blotting (A) followed by densitometry analysis using ImageJ software (B). Tubulin was used as the loading control. (C) miR-21 and its putative binding sequences in the mouse 3'-UTR of Spry2. The binding sequences in the complementary sites were replaced by the indicated nucleotides to produce mutant Spry2 3'-UTR luciferase reporter constructs. Marked sequences represent the conserved complementary nucleotides of the miR-21 binding sequence in humans and various mammals. (D, E) Luciferase activity of the wild-type and mutated 3'-UTR of Spry2 treated with miR-21 mimic (D) or inhibitor (E). *P < 0.05, **P < 0.01, ***P < 0.001, versus the untreated group (B) or mimic NC (D) or inhibitor NC group (E). Experiments were performed in triplicate.
