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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- PAB9985 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9985, RRID:AB_1671742
- Product name
- CBLC polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CBLC.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular cloning and characterization of a novel cbl-family gene, cbl-c.
cbl-3: a new mammalian cbl family protein.
Cloning and characterization of cbl-b: a SH3 binding protein with homology to the c-cbl proto-oncogene.
Kim M, Tezuka T, Suziki Y, Sugano S, Hirai M, Yamamoto T
Gene 1999 Oct 18;239(1):145-54
Gene 1999 Oct 18;239(1):145-54
cbl-3: a new mammalian cbl family protein.
Keane MM, Ettenberg SA, Nau MM, Banerjee P, Cuello M, Penninger J, Lipkowitz S
Oncogene 1999 Jun 3;18(22):3365-75
Oncogene 1999 Jun 3;18(22):3365-75
Cloning and characterization of cbl-b: a SH3 binding protein with homology to the c-cbl proto-oncogene.
Keane MM, Rivero-Lezcano OM, Mitchell JA, Robbins KC, Lipkowitz S
Oncogene 1995 Jun 15;10(12):2367-77
Oncogene 1995 Jun 15;10(12):2367-77
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation and western blot using CBLC polyclonal antibody (Cat # PAB9985) shows detection of a pre-dominant band at ~52 kDa corresponding to CBLC (arrowhead) in transfected cell lysates (left panel).Lysates are from HEK 293T cells transfected with empty vector or with CBLC.The predicted size of CBLC is 52 kDa.Size markers in kDa are shown to the left of the panel.The right panel shows western blotting after first immunoprecipitating with Rabbit anti-CBLC followed by immunoblotting using a Goat anti-CBLC antibody.Personal Communication. Stan Lipkowitz, NCI, NIH, Bethesda, MD.
- Validation comment
- Immunoprecipitation
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry CBLC polyclonal antibody (Cat # PAB9985) shows strong nuclear and cytoplasmic staining of cells in tubuli in human kidney tissue.Tissue was formalin-fixed and paraffin embedded.Brown color indicates presence of protein, blue color shows cell nuclei.Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CBLC polyclonal antibody (Cat # PAB9985) was used at 5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.This image shows moderate intracellular positive staining of human pancreatic acinar epithelium at 40X.Tissue was formalin-fixed and paraffin embedded.The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
