600-401-889

antibody from Invitrogen Antibodies

Targeting: CBLC CBL-3, CBL-SL, RNF57

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-889

Provider

Invitrogen Antibodies

Product name

Cbl-c Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation (right) and western blot (left) using Rocklands Affinity Purified anti-Cbl-c antibody shows detection of a predominant band at ~52 kDa corresponding to Cbl-c. Lysates used are from HEK293T cells transfected with empty vector or with Cbl-c and western blotting (left panel). The predicted size of Cbl-c is 52 kDa. Size markers in kDa are shown to the left of the panel. The (right panel) shows immunoprecipitation with Rabbit anti-Cbl-c followed by western blotting using a Goat anti-Cbl-c antibody. Personal Communication. Stan Lipkowitz, NCI, NIH, Bethesda, MD.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rocklands affinity purified anti-Cbl-c antibody was used at 5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining of human pancreatic acinar epithelium at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rocklands Affinity Purified anti-Cbl-c antibody shows strong nuclear and cytoplasmic staining of cells in tubuli in human kidney tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.