Explore
Validate
LearnPAB11254
antibody from Abnova Corporation
Targeting: RFFL
CARP-2, CARP2, fring, rififylin, RNF189, RNF34L
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB11254 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11254, RRID:AB_1712880
- Product name
- RFFL polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant RFFL.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Over-expression of Rififylin, a new RING finger and FYVE-like domain-containing protein, inhibits recycling from the endocytic recycling compartment.
Crystal structure of a FYVE-type zinc finger domain from the caspase regulator CARP2.
Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth.
Coumailleau F, Das V, Alcover A, Raposo G, Vandormael-Pournin S, Le Bras S, Baldacci P, Dautry-Varsat A, Babinet C, Cohen-Tannoudji M
Molecular biology of the cell 2004 Oct;15(10):4444-56
Molecular biology of the cell 2004 Oct;15(10):4444-56
Crystal structure of a FYVE-type zinc finger domain from the caspase regulator CARP2.
Tibbetts MD, Shiozaki EN, Gu L, McDonald ER 3rd, El-Deiry WS, Shi Y
Structure (London, England : 1993) 2004 Dec;12(12):2257-63
Structure (London, England : 1993) 2004 Dec;12(12):2257-63
Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth.
McDonald ER 3rd, El-Deiry WS
Proceedings of the National Academy of Sciences of the United States of America 2004 Apr 20;101(16):6170-5
Proceedings of the National Academy of Sciences of the United States of America 2004 Apr 20;101(16):6170-5
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using RFFL polyclonal antibody (Cat # PAB11254) shows detection of RFFL (arrowhead) in lysate.Lanes correspond to empty vector 293T cell lysate (1) ; RNF34 transfected lysate (2) and RFFL transfected lysate (3), are shown using 20 uL of lysate per lane.The primary antibody diluted to 1:1,000.Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of RFFL polyclonal antibody (Cat # PAB11254) shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue.Tissue was formalin-fixed and paraffin embedded.Brown color indicates presence of protein, blue color shows cell nuclei.Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
