Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA1-31766 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAD2L2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-31766 detects MAD2L2 from human samples.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAD2L2 using a MAD2L2 polyclonal antibody (Product # PA1-31766).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAD2L2 Polyclonal Antibody (Product # PA1-31766) was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a 20 kDa band corresponding to human MAD2L2 (predicted molecular weight is 24 kDa). Approximately 20 µg of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjµgated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of MAD2L2 was performed in tumor cells of cancerous human kidney tissue using MAD2L2 Polyclonal Antibody (Product # PA1-31766). Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.