Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-49352 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAD2L2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references ALC1 links chromatin accessibility to PARP inhibitor response in homologous recombination-deficient cells.
Verma P, Zhou Y, Cao Z, Deraska PV, Deb M, Arai E, Li W, Shao Y, Puentes L, Li Y, Patankar S, Mach RH, Faryabi RB, Shi J, Greenberg RA
Nature cell biology 2021 Feb;23(2):160-171
Nature cell biology 2021 Feb;23(2):160-171
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAD2L2 in lysates from Hela, K562 cell line (from left to right). Samples were probed with MAD2L2 Antibody (C-term) (Product # PA5-49352) diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of MAD2L2 in Hela cells. Cells were stained with MAD2L2 polyclonal Antibody (C-term) (Product # PA5-49352) at a 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 Phalloidin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MAD2L2 in paraffin-embedded human kidney tissue. Samples were probed with a MAD2L2 Antibody at a 1:25 dilution (Product # PA5-49352) followed by peroxidase-conjugation of the goat anti-rabbit IgG secondary antibody at 1:400 dilution and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 ALC1 loss causes PARPi hypersensitivity in HR-deficient cells. a , Schematic of the dual Cas9 CRISPR system for the GFP competition experiments. b , GFP competition experiment in the UWB1.289+ BRCA1 addback line to examine cell proliferation and ola sensitivity following the combined loss of ALC1 and the indicated DNA repair protein. Data are mean +- s.e.m., normalized to T initial . After every two population doublings, cells were passaged (P) and percent GFP was recorded (n=4 independent transductions, except for sg XRCC1 and sg ERCC4 , where n = 6 independent transductions were made). c , Immunoblot showing ALC1, 53BP1 and Rev7 depletion in UWB1.289 cells. Consistent results were obtained across two independent blots. d , Sensitivities of the indicated UWB1.289 cell lines to ola using the CellTiter-Glo assay; n=3 biologically independent experiments. Data are mean +- s.e.m. e , Immunoblot showing BRCA1 and ALC1 levels in the indicated SUM149PT cells. Consistent results were obtained across two independent blots. f , Sensitivities of the indicated SUM149PT cell lines to ola (left) and talazoparib (tal) (right) using the CellTiter-Glo assay. c#1 and c#2 indicate two different clones with restored BRCA1 reading frames. Data are mean from 2 biologically independent experiments. Source data are provided.
