Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-29540 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SFRP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-29540 detects SFRP1 from human samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE, transferred onto nitrocellulose, and probed using a SFRP1 polyclonal antibody (Product # PA1-29540) at a dilution of 1:230 overnight at 4ºC. Signal was detected using standard techniques.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot using GeneTex Affinity Purified anti-SFRP1 Polyclonal Antibody (Product # PA1-29540) shows detection of a band ~37 kDa (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 Polyclonal Antibody overnight at 4ºC. Signal was detected using standard techniques.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot using GeneTex Affinity Purified anti-SFRP1 Polyclonal Antibody (Product # PA1-29540) shows detection of a band ~37 kDa (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 Polyclonal Antibody overnight at 4ºC. Signal was detected using standard techniques.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SFRP1 Polyclonal Antibody (Product # PA1-29540) was used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue in immunohistochemistry. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG (Product # PA1-28564) is suitable for secondary antibody detection.