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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10235 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10235, RRID:AB_1713866
- Product name
- SFRP1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SFRP1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Evaluation of SFRP1 as a candidate for human retinal dystrophies.
Secreted frizzled-related protein 1 (SFRP1) protects fibroblasts from ceramide-induced apoptosis.
Human secreted frizzled-related protein is down-regulated and induces apoptosis in human cervical cancer.
Garcia-Hoyos M, Cantalapiedra D, Arroyo C, Esteve P, RodrÃguez J, Riveiro R, Trujillo MJ, Ramos C, Bovolenta P, Ayuso C
Molecular vision 2004 Jun 28;10:426-31
Molecular vision 2004 Jun 28;10:426-31
Secreted frizzled-related protein 1 (SFRP1) protects fibroblasts from ceramide-induced apoptosis.
Han X, Amar S
The Journal of biological chemistry 2004 Jan 23;279(4):2832-40
The Journal of biological chemistry 2004 Jan 23;279(4):2832-40
Human secreted frizzled-related protein is down-regulated and induces apoptosis in human cervical cancer.
Ko J, Ryu KS, Lee YH, Na DS, Kim YS, Oh YM, Kim IS, Kim JW
Experimental cell research 2002 Nov 1;280(2):280-7
Experimental cell research 2002 Nov 1;280(2):280-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using SFRP1 polyclonal antibody (Cat # PAB10235) shows detection of a band ~37 KDa band (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells.Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of SFRP1 polyclonal antibody overnight at 4°C.Signal was detected using standard techniques.Personnel communication Becky Mercer, University of Columbia.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of SFRP1 polyclonal antibody (Cat # PAB10235) was used at a 1 : 800 dilution for 20 min to detect SFRP1 in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Goat-anti-Rabbit IgG (p/n 611-1302) is suitable for secondary antibody detection.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
