Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
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- Product number
- PA1-30042 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLI2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted molecular weight of 133 kDa. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Gli2 in HepG2 whole cell lysate using a Gli2 polyclonal antibody (Product # PA1-30042).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GLI2 was performed by loading Jurkat cells. Proteins were transferred to a membrane and probed with a GLI2 Polyclonal Antibody (Product # PA1-30042) at a dilution of 2.5 µg/mL. Lane A : primary antibody, Lane B : primary antibody + blocking peptide
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GLI2 was performed by loading HepG2 cells. Proteins were transferred to a membrane and probed with a GLI2 Polyclonal Antibody (Product # PA1-30042) at a dilution of 1.0 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of GLI2 was performed in mouse gut tissue using GLI2 Polyclonal Antibody (Product # PA1-30042) (green) at a dilution of 1:50. Red : Neural crest cell. Fixed tissue was treated with 0.1% Triton X-100 for 4-6 hours at room temperature or overnight at 4°C, washed in PBS, incubated with primary antibodies for 4-6 hours at room temperature or overnight at 4°C, washed in PBS, and then stained with secondary antibody (Goat Anti-Rabbit Cy3) for 4-6 hours at room temperature or overnight at 4°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of GLI2 was performed in mouse gut tissue using GLI2 Polyclonal Antibody (Product # PA1-30042) (green) at a dilution of 1:50. Red : Neural crest cell. Fixed tissue was treated with 0.1% Triton X-100 for 4-6 hours at room temperature or overnight at 4°C, washed in PBS, incubated with primary antibodies for 4-6 hours at room temperature or overnight at 4°C, washed in PBS, and then stained with secondary antibody (Goat Anti-Rabbit Cy3) for 4-6 hours at room temperature or overnight at 4°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of GLI2 in human heart tissue using GLI2 Polyclonal Antibody (Product # PA1-30042) at a dilution of 4.0-8.0 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of GLI2 in human intestine tissue using GLI2 Polyclonal Antibody (Product # PA1-30042) at a dilution of 4.0-8.0 µg/mL.