Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- AF3635 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal GLI-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse GLI-2 in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling.
A Smo/Gli Multitarget Hedgehog Pathway Inhibitor Impairs Tumor Growth.
Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning.
Itch/β-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis.
TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms.
Suppressor of Fused restraint of Hedgehog activity level is critical for osteogenic proliferation and differentiation during calvarial bone development.
EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.
Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord.
Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-β2 nuclear import system.
Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-α/β1-Independent.
Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling.
Pharmacological GLI2 inhibition prevents myofibroblast cell-cycle progression and reduces kidney fibrosis.
Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators.
The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins.
Martin-Hurtado A, Martin-Morales R, Robledinos-Antón N, Blanco R, Palacios-Blanco I, Lastres-Becker I, Cuadrado A, Garcia-Gonzalo FR
Scientific reports 2019 Sep 25;9(1):13896
Scientific reports 2019 Sep 25;9(1):13896
A Smo/Gli Multitarget Hedgehog Pathway Inhibitor Impairs Tumor Growth.
Lospinoso Severini L, Quaglio D, Basili I, Ghirga F, Bufalieri F, Caimano M, Balducci S, Moretti M, Romeo I, Loricchio E, Maroder M, Botta B, Mori M, Infante P, Di Marcotullio L
Cancers 2019 Oct 9;11(10)
Cancers 2019 Oct 9;11(10)
Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning.
Ma P, Song NN, Li Y, Zhang Q, Zhang L, Zhang L, Kong Q, Ma L, Yang X, Ren B, Li C, Zhao X, Li Y, Xu Y, Gao X, Ding YQ, Mao B
Cell reports 2019 Jul 9;28(2):541-553.e4
Cell reports 2019 Jul 9;28(2):541-553.e4
Itch/β-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis.
Infante P, Faedda R, Bernardi F, Bufalieri F, Lospinoso Severini L, Alfonsi R, Mazzà D, Siler M, Coni S, Po A, Petroni M, Ferretti E, Mori M, De Smaele E, Canettieri G, Capalbo C, Maroder M, Screpanti I, Kool M, Pfister SM, Guardavaccaro D, Gulino A, Di Marcotullio L
Nature communications 2018 Mar 7;9(1):976
Nature communications 2018 Mar 7;9(1):976
TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms.
Rosengren T, Larsen LJ, Pedersen LB, Christensen ST, Møller LB
Cellular and molecular life sciences : CMLS 2018 Jul;75(14):2663-2680
Cellular and molecular life sciences : CMLS 2018 Jul;75(14):2663-2680
Suppressor of Fused restraint of Hedgehog activity level is critical for osteogenic proliferation and differentiation during calvarial bone development.
Li J, Cui Y, Xu J, Wang Q, Yang X, Li Y, Zhang X, Qiu M, Zhang Z, Zhang Z
The Journal of biological chemistry 2017 Sep 22;292(38):15814-15825
The Journal of biological chemistry 2017 Sep 22;292(38):15814-15825
EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.
Guen VJ, Chavarria TE, Kröger C, Ye X, Weinberg RA, Lees JA
Proceedings of the National Academy of Sciences of the United States of America 2017 Dec 5;114(49):E10532-E10539
Proceedings of the National Academy of Sciences of the United States of America 2017 Dec 5;114(49):E10532-E10539
Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord.
Cai H, Liu A
Developmental biology 2017 Dec 1;432(1):72-85
Developmental biology 2017 Dec 1;432(1):72-85
Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-β2 nuclear import system.
Han Y, Xiong Y, Shi X, Wu J, Zhao Y, Jiang J
PLoS biology 2017 Aug;15(8):e2002063
PLoS biology 2017 Aug;15(8):e2002063
Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-α/β1-Independent.
Torrado B, Graña M, Badano JL, Irigoín F
PloS one 2016;11(8):e0162033
PloS one 2016;11(8):e0162033
Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling.
Cai H, Liu A
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 20;113(51):14751-14756
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 20;113(51):14751-14756
Pharmacological GLI2 inhibition prevents myofibroblast cell-cycle progression and reduces kidney fibrosis.
Kramann R, Fleig SV, Schneider RK, Fabian SL, DiRocco DP, Maarouf O, Wongboonsin J, Ikeda Y, Heckl D, Chang SL, Rennke HG, Waikar SS, Humphreys BD
The Journal of clinical investigation 2015 Aug 3;125(8):2935-51
The Journal of clinical investigation 2015 Aug 3;125(8):2935-51
Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators.
Liu YP, Tsai IC, Morleo M, Oh EC, Leitch CC, Massa F, Lee BH, Parker DS, Finley D, Zaghloul NA, Franco B, Katsanis N
The Journal of clinical investigation 2014 May;124(5):2059-70
The Journal of clinical investigation 2014 May;124(5):2059-70
The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins.
Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R
Genes & development 2010 Apr 1;24(7):670-82
Genes & development 2010 Apr 1;24(7):670-82
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- GLI-2 in Mouse Embryo. GLI-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse GLI-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3635) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing muscle. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.