TA804549

antibody from OriGene

Targeting: GLI2 HPE9, THP1, THP2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA804549

Provider

OriGene

Proper citation

OriGene Cat#TA804549, RRID:AB_2627343

Product name

GLI2 mouse monoclonal antibody, clone OTI1F9 (formerly 1F9)

Antibody type

Monoclonal

Description

GLI2 mouse monoclonal antibody, clone OTI1F9 (formerly 1F9)

Host

Mouse

Conjugate

Unconjugated

Epitope

GLI2

Isotype

IgG

Antibody clone number

OTI1F9

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI2 (RC217291, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI2.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-GLI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804549)

Validation comment

IHC