TA319346

antibody from OriGene

Targeting: GLI2 HPE9, THP1, THP2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319346

Provider

OriGene

Product name

Rabbit polyclonal anti-Gli-2 antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal anti-Gli-2 antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

Gli2

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.02 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB using Anti-Gli-2 antibody shows detection of a predominant band at ~190 kDa corresponding to Gli-2 (arrowhead) in mouse brain whole cell lysate (lane 1). Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (lane 2). The primary antibody was diluted to 1:750. Incubation was at RT for 2 h followed by washes and reaction with a 1:10,000 dilution of IRDye? 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Affinity Purified anti-mouse Gli-2 antibody was used at 10 ug/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues, with low background staining. This image shows Gli-2 staining of mouse testis. Tissue was formalin-fixed and paraffin embedded.

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Affinity Purified anti-mouse Gli-2 antibody was used at 10 ug/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues with low background staining. This image shows Gli-2 staining of mouse brain. Tissue was formalin-fixed and paraffin embedded.

Validation comment

IHC