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Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Chromatin Immunoprecipitation [2]
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Validation data
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- Product number
- PA5-47500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLI2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse GLI-2 and 5% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-3 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Chromatin immunoprecipitation of Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-2/DNA complexes were immunoprecipitated using 5 µg Sheep Anti-human GLI-2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47500) or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The bcl-2 promoter was detected by standard PCR.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP assay of GLI2 in Jurkat human acute T cell leukemia cell line. Samples were immunoprecipitated with GLI2 polyclonal antibody (Product # PA5-47500) for 15 minutes in an ultrasonic bath using a dilution of 5 μg followed by a Biotinylated Anti-Sheep IgG secondary antibody. Cell line was treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The bcl-2 promoter was detected by standard PCR.
