711045

antibody from Invitrogen Antibodies

Targeting: GLI2 HPE9, THP1, THP2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711045

Provider

Invitrogen Antibodies

Product name

GLI2 Recombinant Polyclonal Antibody (9HCLC)

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

9HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Nuclear enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with TGF-β1 (Serum starved and treated with 5 ng/mL for 48hr) (Lane 2), MCF-7 (Lane 3), MCF-7 treated with TGF-β1 (Serum starved and treated with 5 ng/mL for 48hr) (Lane 4), A549 (Lane 5), A549 treated with TGF-β1 (Serum starved and treated with 5 ng/mL for 48hr) (Lane 6) and tissue extracts of mouse pancreas (Lane 7), Rat Pancreas (Lane 8) and Mouse Kidney (Lane 9). The blots were probed with Anti-Gli-2 Recombinant Rabbit Polyclonal Antibody (Product # 711045, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Two Isoforms at 168 and 132 kDa band corresponding to Gli-2 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane using wet transfer method. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).