702013

antibody from Invitrogen Antibodies

Targeting: GLI2 HPE9, THP1, THP2

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

702013

Provider

Invitrogen Antibodies

Product name

GLI2 Recombinant Rabbit Monoclonal Antibody (9H25L4)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody is predicted to react with Monkey, Pig, Bovine, Rabbit

Antibody clone number

9H25L4

Concentration

0.5 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, NTERA-2 cells were fixed and permeabilized for detection of endogenous GLI-2 using Anti- GLI-2 Recombinant Rabbit Monoclonal Antibody (Product # 702013, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of GLI-2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating Nuclear localization of GLI-2. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Antibody specificity was demonstrated through detection of enrichment of the target protein at specific gene loci. Chromatin Immunoprecipitation (ChIP) was performed using Anti-GLI-2 Recombinant Rabbit Monoclonal Antibody (Product # 702013) with relevant positive (GLI1) and negative target genes/ binding sites (E2F3, SAT2 satellite repeat)