A00701-5

antibody from Boster Biological Technology

Targeting: GLI2 HPE9, THP1, THP2

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00701-5

Provider

Boster Biological Technology

Product name

Anti-Zinc finger protein GLI2 GLI2 Antibody

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for GLI2 detection. Tested with WB, IHC-P, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Regulator of cullins-1 (ROC1) negatively regulates the Gli2 regulator SUFU to activate the hedgehog pathway in bladder cancer.

Wang W, Qiu J, Qu P, Chen H, Lan J, Chen H, Li L, Gu M
Cancer cell international 2021 Jan 26;21(1):75

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GLI2 induces PDGFRB expression and modulates cancer stem cell properties of gastric cancer.

Wang JX, Zhou JF, Huang FK, Zhang L, He QL, Qian HY, Lai HL
European review for medical and pharmacological sciences 2017 Oct;21(17):3857-3865

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Hedgehog pathway inhibitor-4 suppresses malignant properties of chondrosarcoma cells by disturbing tumor ciliogenesis.

Xiang W, Jiang T, Guo F, Gong C, Yang K, Wu Y, Huang X, Cheng W, Xu K
Oncology reports 2014 Oct;32(4):1622-30

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of GLI2 using anti-GLI2 antibody (A00701-5).GLI2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLI2 Antibody (A00701-5) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image