18989-1-AP

antibody from Proteintech Group

Targeting: GLI2 HPE9, THP1, THP2

Provider product page for 18989-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [48]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 18989-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#18989-1-AP, RRID:AB_10596479
Product name: GLI2-Specific antibody
Antibody type: Polyclonal
Description: KD/KO validated GLI2-Specific antibody (Cat. #18989-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

GLI2 protein structure - 18989-1-AP shown in red.

Submitted references The anti-tumor activity of tangeretin in esophageal squamous cell carcinoma by inhibiting GLI2-mediated transcription of GPNMB.

Yang D, Zhang Q, Kuang H, Liu J, Wu S, Wei L, Yao W
PloS one 2024;19(6):e0291531

Targeted inhibition of the HNF1A/SHH axis by triptolide overcomes paclitaxel resistance in non-small cell lung cancer.

Li LB, Yang LX, Liu L, Liu FR, Li AH, Zhu YL, Wen H, Xue X, Tian ZX, Sun H, Li PC, Zhao XG
Acta pharmacologica Sinica 2024 May;45(5):1060-1076

GLI family zinc finger protein 2 promotes skin fibroblast proliferation and DNA damage repair by targeting the miR-200/ataxia telangiectasia mutated axis in diabetic wound healing.

Liang ZH, Lin SS, Qiu ZY, Pan YC, Pan NF, Liu Y
The Kaohsiung journal of medical sciences 2024 May;40(5):422-434

Ablated Sonic Hedgehog Signaling in the Dentate Gyrus of the Dorsal and Ventral Hippocampus Impairs Hippocampal-Dependent Memory Tasks and Emotion in a Rat Model of Depression.

Luo Y, Wang Y, Qiu F, Hou G, Liu J, Yang H, Wu M, Dong X, Guo D, Zhong Z, Zhang X, Ge J, Meng P
Molecular neurobiology 2024 Jul;61(7):4352-4368

Multifaceted approach toward mapping out the anticancer properties of small molecules via in vitro evaluation on melanoma and nonmelanoma skin cancer cells, and in silico target fishing.

Boateng ST, Roy T, Agbo ME, Mahmud MA, Banang-Mbeumi S, Chamcheu RN, Yadav RK, Bramwell M, Pham LK, Dang DD, Jackson KE, Nagalo BM, Hill RA, Efimova T, Fotie J, Chamcheu JC
Chemical biology & drug design 2024 Jan;103(1):e14418

Molecular mechanism of angiogenesis for cerebral infarction rats by acupuncture intervention based on sonic hedgehog signaling pathway.

Wu HN, Li J, He Y, Georgi R, Kolberg B, Wang SY
Physiology & behavior 2024 Feb 1;274:114420

The GLI2/CDH6 axis enhances migration, invasion and mitochondrial fission of stomach adenocarcinoma cells.

Liu GY, Wang H, Ran R, Wang YC, Li Y
Biochemical and biophysical research communications 2023 Oct 8;676:182-189

Enhanced primary ciliogenesis via mitochondrial oxidative stress activates AKT to prevent neurotoxicity in HSPA9/mortalin-depleted SH-SY5Y cells.

Bae JE, Jang S, Kim JB, Hyung H, Park NY, Kim YH, Kim SH, Kim SH, Ha JM, Oh GS, Park K, Jeong K, Jang JS, Jo DS, Kim P, Lee HS, Ryoo ZY, Cho DH
Molecular brain 2023 May 11;16(1):41

Low-dose phthalates promote breast cancer stem cell properties via the oncogene ΔNp63α and the Sonic hedgehog pathway.

Cao WS, Zhao MJ, Chen Y, Zhu JY, Xie CF, Li XT, Geng SS, Zhong CY, Fu JY, Wu JS
Ecotoxicology and environmental safety 2023 Mar 1;252:114605

Gut microbiota dysbiosis promotes the development of epithelial ovarian cancer via regulating Hedgehog signaling pathway.

Hu X, Xu X, Zeng X, Jin R, Wang S, Jiang H, Tang Y, Chen G, Wei J, Chen T, Chen Q
Gut microbes 2023 Jan-Dec;15(1):2221093

Wdr4 promotes cerebellar development and locomotion through Arhgap17-mediated Rac1 activation.

Wu PR, Chiang SY, Midence R, Kao WC, Lai CL, Cheng IC, Chou SJ, Chen CC, Huang CY, Chen RH
Cell death & disease 2023 Jan 21;14(1):52

1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia.

Li XW, Ran JH, Zhou H, He JZ, Qiu ZW, Wang SY, Wu MN, Zhu S, An YP, Ma A, Li M, Quan YZ, Li NN, Ren CQ, Yang BX
Acta pharmacologica Sinica 2023 Feb;44(2):406-420

A Sonic Hedgehog-Gli-Bmi1 signaling pathway plays a critical role in p27 deficiency induced bone anabolism.

Wu J, Wang R, Kan X, Zhang J, Sun W, Goltzman D, Miao D
International journal of biological sciences 2022;18(3):956-969

Carnitine Protects against MPP(+)-Induced Neurotoxicity and Inflammation by Promoting Primary Ciliogenesis in SH-SY5Y Cells.

Bae JE, Kim JB, Jo DS, Park NY, Kim YH, Lee HJ, Kim SH, Kim SH, Son M, Kim P, Ryu HY, Lee WH, Ryoo ZY, Lee HS, Jung YK, Cho DH
Cells 2022 Sep 1;11(17)

Identification by RNA-Seq of let-7 clusters as prenatal biomarkers for nonsyndromic cleft lip with palate.

Jia S, Zhang Q, Wang Y, Wei X, Gu H, Liu D, Ma W, He Y, Luo W, Yuan Z
Annals of the New York Academy of Sciences 2022 Oct;1516(1):234-246

Rosmarinic Acid Decreases the Malignancy of Pancreatic Cancer Through Inhibiting Gli1 Signaling.

Zhou X, Wang W, Li Z, Chen L, Wen C, Ruan Q, Xu Z, Liu R, Xu J, Bai Y, Deng J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jan;95:153861

Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway.

Cao W, Li Y, Sun H, Yang C, Zhu J, Xie C, Li X, Wu J, Geng S, Wang L, Sun L, Geng G, Han H, Zhong C
Frontiers in cell and developmental biology 2021;9:679806

Resveratrol Inhibition of Renal Cancer Stem Cell Characteristics and Modulation of the Sonic Hedgehog Pathway.

Sun H, Zhang T, Liu R, Cao W, Zhang Z, Liu Z, Qian W, Wang D, Yu D, Zhong C
Nutrition and cancer 2021;73(7):1157-1167

MicroRNA-223 restricts liver fibrosis by inhibiting the TAZ-IHH-GLI2 and PDGF signaling pathways via the crosstalk of multiple liver cell types.

Wang X, Seo W, Park SH, Fu Y, Hwang S, Rodrigues RM, Feng D, Gao B, He Y
International journal of biological sciences 2021;17(4):1153-1167

Hedgehog-Gli2 Signaling Promotes Chemoresistance in Ovarian Cancer Cells by Regulating MDR1.

Wang Q, Wei X, Hu L, Zhuang L, Zhang H, Chen Q
Frontiers in oncology 2021;11:794959

Meningitic Escherichia coli α-hemolysin aggravates blood-brain barrier disruption via targeting TGFβ1-triggered hedgehog signaling.

Fu J, Li L, Huo D, Yang R, Yang B, Xu B, Yang X, Dai M, Tan C, Chen H, Wang X
Molecular brain 2021 Jul 19;14(1):116

Astrocyte-Derived TGFβ1 Facilitates Blood-Brain Barrier Function via Non-Canonical Hedgehog Signaling in Brain Microvascular Endothelial Cells.

Fu J, Li L, Huo D, Zhi S, Yang R, Yang B, Xu B, Zhang T, Dai M, Tan C, Chen H, Wang X
Brain sciences 2021 Jan 8;11(1)

Gli affects the stemness and prognosis of epithelial ovarian cancer via homeobox protein NANOG.

Zhao H, Li N, Pang Y, Zhao J, Wu X
Molecular medicine reports 2021 Feb;23(2)

Benzidine promotes the stemness of bladder cancer stem cells via activation of the Sonic hedgehog pathway.

Wang D, Xie D, Bi L, Wang Y, Zou C, Chen L, Geng H, Qian W, Li Y, Sun H, Wang X, Lu Y, Yu D, Zhong C
Oncology letters 2021 Feb;21(2):146

Decreased Indian hedgehog signaling activates autophagy in endometriosis and adenomyosis.

Zhou Y, Peng Y, Xia Q, Yan D, Zhang H, Zhang L, Chen Y, Zhao X, Li J
Reproduction (Cambridge, England) 2021 Feb;161(2):99-109

Cigarette smoke supports stemness and epithelial-mesenchymal transition in bladder cancer stem cells through SHH signaling.

Sun X, Song J, Li E, Geng H, Li Y, Yu D, Zhong C
International journal of clinical and experimental pathology 2020;13(6):1333-1348

HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells.

Arasu UT, Deen AJ, Pasonen-Seppänen S, Heikkinen S, Lalowski M, Kärnä R, Härkönen K, Mäkinen P, Lázaro-Ibáñez E, Siljander PR, Oikari S, Levonen AL, Rilla K
Cellular and molecular life sciences : CMLS 2020 Oct;77(20):4093-4115

Range of SHH signaling in adrenal gland is limited by membrane contact to cells with primary cilia.

Mateska I, Nanda K, Dye NA, Alexaki VI, Eaton S
The Journal of cell biology 2020 Dec 7;219(12)

Developmental mechanisms driving complex tooth shape in reptiles.

Landova Sulcova M, Zahradnicek O, Dumkova J, Dosedelova H, Krivanek J, Hampl M, Kavkova M, Zikmund T, Gregorovicova M, Sedmera D, Kaiser J, Tucker AS, Buchtova M
Developmental dynamics : an official publication of the American Association of Anatomists 2020 Apr;249(4):441-464

CBX2 Regulates Proliferation and Apoptosis via the Phosphorylation of YAP in Hepatocellular Carcinoma.

Mao J, Tian Y, Wang C, Jiang K, Li R, Yao Y, Zhang R, Sun D, Liang R, Gao Z, Wang Q, Wang L
Journal of Cancer 2019;10(12):2706-2719

Sonic hedgehog pathway mediates genistein inhibition of renal cancer stem cells.

Li E, Zhang T, Sun X, Li Y, Geng H, Yu D, Zhong C
Oncology letters 2019 Sep;18(3):3081-3091

Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer.

Adams CR, Htwe HH, Marsh T, Wang AL, Montoya ML, Subbaraj L, Tward AD, Bardeesy N, Perera RM
eLife 2019 May 28;8

Combined inhibition of sonic Hedgehog signaling and histone deacetylase is an effective treatment for liver cancer.

Li J, Cai H, Li H, Liu Y, Wang Y, Shi Y, Sun Y, Song H, Wang D
Oncology reports 2019 Mar;41(3):1991-1997

miR-384-5p Targets Gli2 and Negatively Regulates Age-Related Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells.

Li X, Wu J, Liu S, Zhang K, Miao X, Li J, Shi Z, Gao Y
Stem cells and development 2019 Jun 15;28(12):791-798

Inhibition of MicroRNA-195 Alleviates Neuropathic Pain by Targeting Patched1 and Inhibiting SHH Signaling Pathway Activation.

Wang X, Wang H, Zhang T, He M, Liang H, Wang H, Xu L, Chen S, Xu M
Neurochemical research 2019 Jul;44(7):1690-1702

Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.

Hayes CS, Labuzan SA, Menke JA, Haddock AN, Waddell DS
Journal of cellular physiology 2019 Dec;234(12):23807-23824

Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.

Ge M, Zhang L, Cao L, Xie C, Li X, Li Y, Meng Y, Chen Y, Wang X, Chen J, Zhang Q, Shao J, Zhong C
International journal of food sciences and nutrition 2019 Aug;70(5):570-578

Cigarette smoke stimulates the stemness of renal cancer stem cells via Sonic Hedgehog pathway.

Qian W, Kong X, Zhang T, Wang D, Song J, Li Y, Li X, Geng H, Min J, Kong Q, Liu J, Liu Z, Wang D, Zhang Z, Yu D, Zhong C
Oncogenesis 2018 Mar 13;7(3):24

Low-intensity pulsed ultrasound stimulation promotes osteoblast differentiation through hedgehog signaling.

Matsumoto K, Shimo T, Kurio N, Okui T, Ibaragi S, Kunisada Y, Obata K, Masui M, Pai P, Horikiri Y, Yamanaka N, Takigawa M, Sasaki A
Journal of cellular biochemistry 2018 Jun;119(6):4352-4360

Effects of baicalein on pancreatic cancer stem cells via modulation of sonic Hedgehog pathway.

Song L, Chen X, Wang P, Gao S, Qu C, Liu L
Acta biochimica et biophysica Sinica 2018 Jun 1;50(6):586-596

Disulfiram/copper targets stem cell-like ALDH(+) population of multiple myeloma by inhibition of ALDH1A1 and Hedgehog pathway.

Jin N, Zhu X, Cheng F, Zhang L
Journal of cellular biochemistry 2018 Aug;119(8):6882-6893

Expression patterns of key Sonic Hedgehog signaling pathway components in the developing and adult mouse midbrain and in the MN9D cell line.

Feuerstein M, Chleilat E, Khakipoor S, Michailidis K, Ophoven C, Roussa E
Cell and tissue research 2017 Nov;370(2):211-225

Curcumin inhibits bladder cancer stem cells by suppressing Sonic Hedgehog pathway.

Wang D, Kong X, Li Y, Qian W, Ma J, Wang D, Yu D, Zhong C
Biochemical and biophysical research communications 2017 Nov 4;493(1):521-527

Curcumin Suppresses Lung Cancer Stem Cells via Inhibiting Wnt/β-catenin and Sonic Hedgehog Pathways.

Zhu JY, Yang X, Chen Y, Jiang Y, Wang SJ, Li Y, Wang XQ, Meng Y, Zhu MM, Ma X, Huang C, Wu R, Xie CF, Li XT, Geng SS, Wu JS, Zhong CY, Han HY
Phytotherapy research : PTR 2017 Apr;31(4):680-688

Primary Cilia Negatively Regulate Melanogenesis in Melanocytes and Pigmentation in a Human Skin Model.

Choi H, Shin JH, Kim ES, Park SJ, Bae IH, Jo YK, Jeong IY, Kim HJ, Lee Y, Park HC, Jeon HB, Kim KW, Lee TR, Cho DH
PloS one 2016;11(12):e0168025

GANT61, a GLI inhibitor, sensitizes glioma cells to the temozolomide treatment.

Li J, Cai J, Zhao S, Yao K, Sun Y, Li Y, Chen L, Li R, Zhai X, Zhang J, Jiang C
Journal of experimental & clinical cancer research : CR 2016 Nov 28;35(1):184

Effect of Smo SiRNA-mediated Hedgehog Signaling Pathway Inhibition on Palatal Fusion.

Zhou Q, Wu HF, Wei Y, Zhu WL
Biomedical and environmental sciences : BES 2016 Aug;29(8):594-598

Expression pattern of sonic hedgehog signaling and calcitonin gene-related peptide in the socket healing process after tooth extraction.

Pang P, Shimo T, Takada H, Matsumoto K, Yoshioka N, Ibaragi S, Sasaki A
Biochemical and biophysical research communications 2015 Nov 6;467(1):21-6

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 18989-1-AP(GLI2-Specific antibody) at dilution of 1:400
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using Gli2 antibody 18989-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 18989-1-AP(GLI2-Specific antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 18989-1-AP(GLI2-Specific antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue