Antibody data
- Antibody Data
- Antigen structure
- References [29]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 18989-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#18989-1-AP, RRID:AB_10596479
- Product name
- GLI2-Specific antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated GLI2-Specific antibody (Cat. #18989-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway.
Resveratrol Inhibition of Renal Cancer Stem Cell Characteristics and Modulation of the Sonic Hedgehog Pathway.
MicroRNA-223 restricts liver fibrosis by inhibiting the TAZ-IHH-GLI2 and PDGF signaling pathways via the crosstalk of multiple liver cell types.
Meningitic Escherichia coli α-hemolysin aggravates blood-brain barrier disruption via targeting TGFβ1-triggered hedgehog signaling.
Astrocyte-Derived TGFβ1 Facilitates Blood-Brain Barrier Function via Non-Canonical Hedgehog Signaling in Brain Microvascular Endothelial Cells.
Gli affects the stemness and prognosis of epithelial ovarian cancer via homeobox protein NANOG.
Benzidine promotes the stemness of bladder cancer stem cells via activation of the Sonic hedgehog pathway.
Cigarette smoke supports stemness and epithelial-mesenchymal transition in bladder cancer stem cells through SHH signaling.
HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells.
Range of SHH signaling in adrenal gland is limited by membrane contact to cells with primary cilia.
Developmental mechanisms driving complex tooth shape in reptiles.
CBX2 Regulates Proliferation and Apoptosis via the Phosphorylation of YAP in Hepatocellular Carcinoma.
Sonic hedgehog pathway mediates genistein inhibition of renal cancer stem cells.
Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer.
miR-384-5p Targets Gli2 and Negatively Regulates Age-Related Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells.
Inhibition of MicroRNA-195 Alleviates Neuropathic Pain by Targeting Patched1 and Inhibiting SHH Signaling Pathway Activation.
Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.
Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.
Cigarette smoke stimulates the stemness of renal cancer stem cells via Sonic Hedgehog pathway.
Low-intensity pulsed ultrasound stimulation promotes osteoblast differentiation through hedgehog signaling.
Effects of baicalein on pancreatic cancer stem cells via modulation of sonic Hedgehog pathway.
Disulfiram/copper targets stem cell-like ALDH+ population of multiple myeloma by inhibition of ALDH1A1 and Hedgehog pathway.
Expression patterns of key Sonic Hedgehog signaling pathway components in the developing and adult mouse midbrain and in the MN9D cell line.
Curcumin inhibits bladder cancer stem cells by suppressing Sonic Hedgehog pathway.
Curcumin Suppresses Lung Cancer Stem Cells via Inhibiting Wnt/β-catenin and Sonic Hedgehog Pathways.
Primary Cilia Negatively Regulate Melanogenesis in Melanocytes and Pigmentation in a Human Skin Model.
GANT61, a GLI inhibitor, sensitizes glioma cells to the temozolomide treatment.
Effect of Smo SiRNA-mediated Hedgehog Signaling Pathway Inhibition on Palatal Fusion.
Expression pattern of sonic hedgehog signaling and calcitonin gene-related peptide in the socket healing process after tooth extraction.
Cao W, Li Y, Sun H, Yang C, Zhu J, Xie C, Li X, Wu J, Geng S, Wang L, Sun L, Geng G, Han H, Zhong C
Frontiers in cell and developmental biology 2021;9:679806
Frontiers in cell and developmental biology 2021;9:679806
Resveratrol Inhibition of Renal Cancer Stem Cell Characteristics and Modulation of the Sonic Hedgehog Pathway.
Sun H, Zhang T, Liu R, Cao W, Zhang Z, Liu Z, Qian W, Wang D, Yu D, Zhong C
Nutrition and cancer 2021;73(7):1157-1167
Nutrition and cancer 2021;73(7):1157-1167
MicroRNA-223 restricts liver fibrosis by inhibiting the TAZ-IHH-GLI2 and PDGF signaling pathways via the crosstalk of multiple liver cell types.
Wang X, Seo W, Park SH, Fu Y, Hwang S, Rodrigues RM, Feng D, Gao B, He Y
International journal of biological sciences 2021;17(4):1153-1167
International journal of biological sciences 2021;17(4):1153-1167
Meningitic Escherichia coli α-hemolysin aggravates blood-brain barrier disruption via targeting TGFβ1-triggered hedgehog signaling.
Fu J, Li L, Huo D, Yang R, Yang B, Xu B, Yang X, Dai M, Tan C, Chen H, Wang X
Molecular brain 2021 Jul 19;14(1):116
Molecular brain 2021 Jul 19;14(1):116
Astrocyte-Derived TGFβ1 Facilitates Blood-Brain Barrier Function via Non-Canonical Hedgehog Signaling in Brain Microvascular Endothelial Cells.
Fu J, Li L, Huo D, Zhi S, Yang R, Yang B, Xu B, Zhang T, Dai M, Tan C, Chen H, Wang X
Brain sciences 2021 Jan 8;11(1)
Brain sciences 2021 Jan 8;11(1)
Gli affects the stemness and prognosis of epithelial ovarian cancer via homeobox protein NANOG.
Zhao H, Li N, Pang Y, Zhao J, Wu X
Molecular medicine reports 2021 Feb;23(2)
Molecular medicine reports 2021 Feb;23(2)
Benzidine promotes the stemness of bladder cancer stem cells via activation of the Sonic hedgehog pathway.
Wang D, Xie D, Bi L, Wang Y, Zou C, Chen L, Geng H, Qian W, Li Y, Sun H, Wang X, Lu Y, Yu D, Zhong C
Oncology letters 2021 Feb;21(2):146
Oncology letters 2021 Feb;21(2):146
Cigarette smoke supports stemness and epithelial-mesenchymal transition in bladder cancer stem cells through SHH signaling.
Sun X, Song J, Li E, Geng H, Li Y, Yu D, Zhong C
International journal of clinical and experimental pathology 2020;13(6):1333-1348
International journal of clinical and experimental pathology 2020;13(6):1333-1348
HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells.
Arasu UT, Deen AJ, Pasonen-Seppänen S, Heikkinen S, Lalowski M, Kärnä R, Härkönen K, Mäkinen P, Lázaro-Ibáñez E, Siljander PR, Oikari S, Levonen AL, Rilla K
Cellular and molecular life sciences : CMLS 2020 Oct;77(20):4093-4115
Cellular and molecular life sciences : CMLS 2020 Oct;77(20):4093-4115
Range of SHH signaling in adrenal gland is limited by membrane contact to cells with primary cilia.
Mateska I, Nanda K, Dye NA, Alexaki VI, Eaton S
The Journal of cell biology 2020 Dec 7;219(12)
The Journal of cell biology 2020 Dec 7;219(12)
Developmental mechanisms driving complex tooth shape in reptiles.
Landova Sulcova M, Zahradnicek O, Dumkova J, Dosedelova H, Krivanek J, Hampl M, Kavkova M, Zikmund T, Gregorovicova M, Sedmera D, Kaiser J, Tucker AS, Buchtova M
Developmental dynamics : an official publication of the American Association of Anatomists 2020 Apr;249(4):441-464
Developmental dynamics : an official publication of the American Association of Anatomists 2020 Apr;249(4):441-464
CBX2 Regulates Proliferation and Apoptosis via the Phosphorylation of YAP in Hepatocellular Carcinoma.
Mao J, Tian Y, Wang C, Jiang K, Li R, Yao Y, Zhang R, Sun D, Liang R, Gao Z, Wang Q, Wang L
Journal of Cancer 2019;10(12):2706-2719
Journal of Cancer 2019;10(12):2706-2719
Sonic hedgehog pathway mediates genistein inhibition of renal cancer stem cells.
Li E, Zhang T, Sun X, Li Y, Geng H, Yu D, Zhong C
Oncology letters 2019 Sep;18(3):3081-3091
Oncology letters 2019 Sep;18(3):3081-3091
Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer.
Adams CR, Htwe HH, Marsh T, Wang AL, Montoya ML, Subbaraj L, Tward AD, Bardeesy N, Perera RM
eLife 2019 May 28;8
eLife 2019 May 28;8
miR-384-5p Targets Gli2 and Negatively Regulates Age-Related Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells.
Li X, Wu J, Liu S, Zhang K, Miao X, Li J, Shi Z, Gao Y
Stem cells and development 2019 Jun 15;28(12):791-798
Stem cells and development 2019 Jun 15;28(12):791-798
Inhibition of MicroRNA-195 Alleviates Neuropathic Pain by Targeting Patched1 and Inhibiting SHH Signaling Pathway Activation.
Wang X, Wang H, Zhang T, He M, Liang H, Wang H, Xu L, Chen S, Xu M
Neurochemical research 2019 Jul;44(7):1690-1702
Neurochemical research 2019 Jul;44(7):1690-1702
Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.
Hayes CS, Labuzan SA, Menke JA, Haddock AN, Waddell DS
Journal of cellular physiology 2019 Dec;234(12):23807-23824
Journal of cellular physiology 2019 Dec;234(12):23807-23824
Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.
Ge M, Zhang L, Cao L, Xie C, Li X, Li Y, Meng Y, Chen Y, Wang X, Chen J, Zhang Q, Shao J, Zhong C
International journal of food sciences and nutrition 2019 Aug;70(5):570-578
International journal of food sciences and nutrition 2019 Aug;70(5):570-578
Cigarette smoke stimulates the stemness of renal cancer stem cells via Sonic Hedgehog pathway.
Qian W, Kong X, Zhang T, Wang D, Song J, Li Y, Li X, Geng H, Min J, Kong Q, Liu J, Liu Z, Wang D, Zhang Z, Yu D, Zhong C
Oncogenesis 2018 Mar 13;7(3):24
Oncogenesis 2018 Mar 13;7(3):24
Low-intensity pulsed ultrasound stimulation promotes osteoblast differentiation through hedgehog signaling.
Matsumoto K, Shimo T, Kurio N, Okui T, Ibaragi S, Kunisada Y, Obata K, Masui M, Pai P, Horikiri Y, Yamanaka N, Takigawa M, Sasaki A
Journal of cellular biochemistry 2018 Jun;119(6):4352-4360
Journal of cellular biochemistry 2018 Jun;119(6):4352-4360
Effects of baicalein on pancreatic cancer stem cells via modulation of sonic Hedgehog pathway.
Song L, Chen X, Wang P, Gao S, Qu C, Liu L
Acta biochimica et biophysica Sinica 2018 Jun 1;50(6):586-596
Acta biochimica et biophysica Sinica 2018 Jun 1;50(6):586-596
Disulfiram/copper targets stem cell-like ALDH+ population of multiple myeloma by inhibition of ALDH1A1 and Hedgehog pathway.
Jin N, Zhu X, Cheng F, Zhang L
Journal of cellular biochemistry 2018 Aug;119(8):6882-6893
Journal of cellular biochemistry 2018 Aug;119(8):6882-6893
Expression patterns of key Sonic Hedgehog signaling pathway components in the developing and adult mouse midbrain and in the MN9D cell line.
Feuerstein M, Chleilat E, Khakipoor S, Michailidis K, Ophoven C, Roussa E
Cell and tissue research 2017 Nov;370(2):211-225
Cell and tissue research 2017 Nov;370(2):211-225
Curcumin inhibits bladder cancer stem cells by suppressing Sonic Hedgehog pathway.
Wang D, Kong X, Li Y, Qian W, Ma J, Wang D, Yu D, Zhong C
Biochemical and biophysical research communications 2017 Nov 4;493(1):521-527
Biochemical and biophysical research communications 2017 Nov 4;493(1):521-527
Curcumin Suppresses Lung Cancer Stem Cells via Inhibiting Wnt/β-catenin and Sonic Hedgehog Pathways.
Zhu JY, Yang X, Chen Y, Jiang Y, Wang SJ, Li Y, Wang XQ, Meng Y, Zhu MM, Ma X, Huang C, Wu R, Xie CF, Li XT, Geng SS, Wu JS, Zhong CY, Han HY
Phytotherapy research : PTR 2017 Apr;31(4):680-688
Phytotherapy research : PTR 2017 Apr;31(4):680-688
Primary Cilia Negatively Regulate Melanogenesis in Melanocytes and Pigmentation in a Human Skin Model.
Choi H, Shin JH, Kim ES, Park SJ, Bae IH, Jo YK, Jeong IY, Kim HJ, Lee Y, Park HC, Jeon HB, Kim KW, Lee TR, Cho DH
PloS one 2016;11(12):e0168025
PloS one 2016;11(12):e0168025
GANT61, a GLI inhibitor, sensitizes glioma cells to the temozolomide treatment.
Li J, Cai J, Zhao S, Yao K, Sun Y, Li Y, Chen L, Li R, Zhai X, Zhang J, Jiang C
Journal of experimental & clinical cancer research : CR 2016 Nov 28;35(1):184
Journal of experimental & clinical cancer research : CR 2016 Nov 28;35(1):184
Effect of Smo SiRNA-mediated Hedgehog Signaling Pathway Inhibition on Palatal Fusion.
Zhou Q, Wu HF, Wei Y, Zhu WL
Biomedical and environmental sciences : BES 2016 Aug;29(8):594-598
Biomedical and environmental sciences : BES 2016 Aug;29(8):594-598
Expression pattern of sonic hedgehog signaling and calcitonin gene-related peptide in the socket healing process after tooth extraction.
Pang P, Shimo T, Takada H, Matsumoto K, Yoshioka N, Ibaragi S, Sasaki A
Biochemical and biophysical research communications 2015 Nov 6;467(1):21-6
Biochemical and biophysical research communications 2015 Nov 6;467(1):21-6
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 18989-1-AP(GLI2-Specific antibody) at dilution of 1:400
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using Gli2 antibody 18989-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 18989-1-AP(GLI2-Specific antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 18989-1-AP(GLI2-Specific antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue