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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-2815 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2815, RRID:AB_2111898
- Product name
- Goat Polyclonal GLI-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise all four reported isoforms (NP_084655.1, NP_084656.1, NP_084657.1 and NP_005261.1)
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Gli2 and Gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function.
Haycraft CJ, Banizs B, Aydin-Son Y, Zhang Q, Michaud EJ, Yoder BK
PLoS genetics 2005 Oct;1(4):e53
PLoS genetics 2005 Oct;1(4):e53
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GLI-2 Antibody [NB100-2815] - (4ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GLI-2 Antibody [NB100-2815] - Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.
