AP09261PU-N

antibody from Acris Antibodies GmbH

Targeting: GLI2 HPE9, THP1, THP2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09261PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09261PU-N, RRID:AB_2035558

Product name

anti GLI2

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2

Reactivity

Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.02 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using AP09261PU-N Gli2 antibody shows detection of a predominant band at ~190 kDa corresponding to Gli-2 (arrowhead) in Mouse brain whole cell lysate (Lane 1). Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (Lane 2). ~ 25 μg of lysate was resolved on a 4-8% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1/750. Incubation was at RT for 2 h followed by washes and reaction with a 1/10,000 dilution of IRDye® 800 conjugated Goat anti-Rabbit IgG (H&L) MX10 for 45 min at RT. Molecular weight markers are shown (M) using the 700 nm channel (red). IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP09261PU-N Gli2 antibody staining of Formalin-Fixed, Paraffin Embedded of several Mouse Tesis tissues at 10 μg/ml . Moderate to strong staining was seen on many tissues, with low background staining.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP09261PU-N Gli2 antibody staining of Formalin-Fixed, Paraffin Embedded of several Mouse Brain tissues at 10 μg/ml . Moderate to strong staining was seen on many tissues, with low background staining.