AP09264PU-N

antibody from Acris Antibodies GmbH

Targeting: GLI2 HPE9, THP1, THP2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09264PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09264PU-N, RRID:AB_2035559

Product name

anti GLI2 (40-60)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 46-60 of Human Gli-2 (isoform alpha).

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.1 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western blot using AP09264PU-N Gli-2 antibody shows detection of Gli-2 protein in Rat testes (Lane 1) and Human HEK293 (Lane 2)whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 μg of lysate. Primary antibody was used at a 1/400 dilution in 5% BLOTTO in PBS overnight at 4°C. The membrane was washed and reacted with a 1/10,000 dilution of IRDye® 800 conjugated Goat anti-Rabbit IgG [H&L] for 45 min at RT (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. AP09264PU-N Gli2 antibody staining of Formalin-Fixed, Paraffin Embedded Human breast tissue (strong cytoplasmic and membranous satining). Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.