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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- SC-56 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-56, RRID:AB_628110
- Product name
- Anti-PCNA
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references The base excision repair pathway is required for efficient lentivirus integration.
RB deletion disrupts coordination between DNA replication licensing and mitotic entry in vivo.
Placentation in Sigmodontinae: a rodent taxon native to South America.
Cooperative signaling between Wnt1 and integrin-linked kinase induces accelerated breast tumor development.
Malignant transformation in a defined genetic background: proteome changes displayed by 2D-PAGE.
Lynch syndrome-associated mutations in MSH2 alter DNA repair and checkpoint response functions in vivo.
Role of the ubiquitin-binding domain of Polη in Rad18-independent translesion DNA synthesis in human cell extracts.
Three prime exonuclease I (TREX1) is Fos/AP-1 regulated by genotoxic stress and protects against ultraviolet light and benzo(a)pyrene-induced DNA damage.
Phosphorylated Rad 18 directs DNA Polymerase {eta} to sites of stalled replication.
WRN participates in translesion synthesis pathway through interaction with NBS1.
Tandem E2F binding sites in the promoter of the p107 cell cycle regulator control p107 expression and its cellular functions.
Mitotic chromosome condensation mediated by the retinoblastoma protein is tumor-suppressive.
DNA damage and cell cycle events implicate cerebellar dentate nucleus neurons as targets of Alzheimer's disease.
Induction of cell cycle arrest at G1 and S phases and cAMP-dependent differentiation in C6 glioma by low concentration of cycloheximide.
c-Myc-induced aberrant DNA synthesis and activation of DNA damage response in p300 knockdown cells.
Differential p53-independent outcomes of p19(Arf) loss in oncogenesis.
E7 oncoprotein of novel human papillomavirus type 108 lacking the E6 gene induces dysplasia in organotypic keratinocyte cultures.
The Wilms' tumour suppressor WT1 is involved in endothelial cell proliferation and migration: expression in tumour vessels in vivo
Interaction of estrogen receptor alpha with proliferating cell nuclear antigen.
Rad18 regulates DNA polymerase kappa and is required for recovery from S-phase checkpoint-mediated arrest.
Expression of Dmp1 in specific differentiated, nonproliferating cells and its regulation by E2Fs.
Yoder KE, Espeseth A, Wang XH, Fang Q, Russo MT, Lloyd RS, Hazuda D, Sobol RW, Fishel R
PloS one 2011 Mar 23;6(3):e17862
PloS one 2011 Mar 23;6(3):e17862
RB deletion disrupts coordination between DNA replication licensing and mitotic entry in vivo.
Bourgo RJ, Ehmer U, Sage J, Knudsen ES
Molecular biology of the cell 2011 Apr;22(7):931-9
Molecular biology of the cell 2011 Apr;22(7):931-9
Placentation in Sigmodontinae: a rodent taxon native to South America.
Favaron PO, Carter AM, Ambrósio CE, Morini AC, Mess AM, de Oliveira MF, Miglino MA
Reproductive biology and endocrinology : RB&E 2011 Apr 25;9:55
Reproductive biology and endocrinology : RB&E 2011 Apr 25;9:55
Cooperative signaling between Wnt1 and integrin-linked kinase induces accelerated breast tumor development.
Oloumi A, Maidan M, Lock FE, Tearle H, McKinney S, Muller WJ, Aparicio SA, Dedhar S
Breast cancer research : BCR 2010;12(3):R38
Breast cancer research : BCR 2010;12(3):R38
Malignant transformation in a defined genetic background: proteome changes displayed by 2D-PAGE.
Pütz SM, Vogiatzi F, Stiewe T, Sickmann A
Molecular cancer 2010 Sep 22;9:254
Molecular cancer 2010 Sep 22;9:254
Lynch syndrome-associated mutations in MSH2 alter DNA repair and checkpoint response functions in vivo.
Mastrocola AS, Heinen CD
Human mutation 2010 Oct;31(10):E1699-708
Human mutation 2010 Oct;31(10):E1699-708
Role of the ubiquitin-binding domain of Polη in Rad18-independent translesion DNA synthesis in human cell extracts.
Schmutz V, Janel-Bintz R, Wagner J, Biard D, Shiomi N, Fuchs RP, Cordonnier AM
Nucleic acids research 2010 Oct 1;38(19):6456-65
Nucleic acids research 2010 Oct 1;38(19):6456-65
Three prime exonuclease I (TREX1) is Fos/AP-1 regulated by genotoxic stress and protects against ultraviolet light and benzo(a)pyrene-induced DNA damage.
Christmann M, Tomicic MT, Aasland D, Berdelle N, Kaina B
Nucleic acids research 2010 Oct 1;38(19):6418-32
Nucleic acids research 2010 Oct 1;38(19):6418-32
Phosphorylated Rad 18 directs DNA Polymerase {eta} to sites of stalled replication.
Day TA, Palle K, Barkley LR, Kakusho N, Zou Y, Tateishi S, Verreault A, Masai H, Vaziri C
The Journal of cell biology 2010 Nov 29;191(5):953-66
The Journal of cell biology 2010 Nov 29;191(5):953-66
WRN participates in translesion synthesis pathway through interaction with NBS1.
Kobayashi J, Okui M, Asaithamby A, Burma S, Chen BP, Tanimoto K, Matsuura S, Komatsu K, Chen DJ
Mechanisms of ageing and development 2010 Jun;131(6):436-44
Mechanisms of ageing and development 2010 Jun;131(6):436-44
Tandem E2F binding sites in the promoter of the p107 cell cycle regulator control p107 expression and its cellular functions.
Burkhart DL, Wirt SE, Zmoos AF, Kareta MS, Sage J
PLoS genetics 2010 Jun 24;6(6):e1001003
PLoS genetics 2010 Jun 24;6(6):e1001003
Mitotic chromosome condensation mediated by the retinoblastoma protein is tumor-suppressive.
Coschi CH, Martens AL, Ritchie K, Francis SM, Chakrabarti S, Berube NG, Dick FA
Genes & development 2010 Jul 1;24(13):1351-63
Genes & development 2010 Jul 1;24(13):1351-63
DNA damage and cell cycle events implicate cerebellar dentate nucleus neurons as targets of Alzheimer's disease.
Chen J, Cohen ML, Lerner AJ, Yang Y, Herrup K
Molecular neurodegeneration 2010 Dec 20;5:60
Molecular neurodegeneration 2010 Dec 20;5:60
Induction of cell cycle arrest at G1 and S phases and cAMP-dependent differentiation in C6 glioma by low concentration of cycloheximide.
Liu X, Yang JM, Zhang SS, Liu XY, Liu DX
BMC cancer 2010 Dec 15;10:684
BMC cancer 2010 Dec 15;10:684
c-Myc-induced aberrant DNA synthesis and activation of DNA damage response in p300 knockdown cells.
Sankar N, Kadeppagari RK, Thimmapaya B
The Journal of biological chemistry 2009 May 29;284(22):15193-205
The Journal of biological chemistry 2009 May 29;284(22):15193-205
Differential p53-independent outcomes of p19(Arf) loss in oncogenesis.
Chen Z, Carracedo A, Lin HK, Koutcher JA, Behrendt N, Egia A, Alimonti A, Carver BS, Gerald W, Teruya-Feldstein J, Loda M, Pandolfi PP
Science signaling 2009 Aug 18;2(84):ra44
Science signaling 2009 Aug 18;2(84):ra44
E7 oncoprotein of novel human papillomavirus type 108 lacking the E6 gene induces dysplasia in organotypic keratinocyte cultures.
Nobre RJ, Herráez-Hernández E, Fei JW, Langbein L, Kaden S, Gröne HJ, de Villiers EM
Journal of virology 2009 Apr;83(7):2907-16
Journal of virology 2009 Apr;83(7):2907-16
The Wilms' tumour suppressor WT1 is involved in endothelial cell proliferation and migration: expression in tumour vessels in vivo
N Wagner, J F Michiels, A Schedl, K-D Wagner
Oncogene 2008 Jan;27(26):3662-3672
Oncogene 2008 Jan;27(26):3662-3672
Interaction of estrogen receptor alpha with proliferating cell nuclear antigen.
Schultz-Norton JR, Gabisi VA, Ziegler YS, McLeod IX, Yates JR, Nardulli AM
Nucleic acids research 2007;35(15):5028-38
Nucleic acids research 2007;35(15):5028-38
Rad18 regulates DNA polymerase kappa and is required for recovery from S-phase checkpoint-mediated arrest.
Bi X, Barkley LR, Slater DM, Tateishi S, Yamaizumi M, Ohmori H, Vaziri C
Molecular and cellular biology 2006 May;26(9):3527-40
Molecular and cellular biology 2006 May;26(9):3527-40
Expression of Dmp1 in specific differentiated, nonproliferating cells and its regulation by E2Fs.
Mallakin A, Taneja P, Matise LA, Willingham MC, Inoue K
Oncogene 2006 Dec 14;25(59):7703-13
Oncogene 2006 Dec 14;25(59):7703-13
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 3
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
