Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [30]
- Comments [0]
- Validations
- Western blot [11]
- Immunocytochemistry [3]
- Immunoprecipitation [1]
- Immunohistochemistry [5]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX100539 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100539, RRID:AB_1241163
- Product name
- PCNA antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Strand break-induced replication fork collapse leads to C-circles, C-overhangs and telomeric recombination.
Preprogramming therapeutic response of PI3K/mTOR dual inhibitor via the regulation of EHMT2 and p27 in pancreatic cancer.
Neuroprotection against 6-OHDA toxicity in PC12 cells and mice through the Nrf2 pathway by a sesquiterpenoid from Tussilago farfara.
A high-throughput pipeline for validation of antibodies.
Lactobacillus paracasei CNCM I-3689 reduces vancomycin-resistant Enterococcus persistence and promotes Bacteroidetes resilience in the gut following antibiotic challenge.
Bisdemethoxycurcumin Suppresses Migration and Invasion of Human Cervical Cancer HeLa Cells via Inhibition of NF-ĸB, MMP-2 and -9 Pathways.
EZH2 inhibits autophagic cell death of aortic vascular smooth muscle cells to affect aortic dissection.
Exosomal MicroRNA-10a Is Associated with Liver Regeneration in Rats through Downregulation of EphA4.
Overexpression of PCNA Attenuates Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced DNA Damage.
A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
CD24+ tumor-initiating cells from oral squamous cell carcinoma induce initial angiogenesis in vivo.
Preventive effect of celecoxib use against cancer progression and occurrence of oral squamous cell carcinoma.
Progression-related loss of stromal Caveolin 1 levels fosters the growth of human PC3 xenografts and mediates radiation resistance.
Adipose-derived mesenchymal stem cells promote liver regeneration and suppress rejection in small-for-size liver allograft.
Looping-out mechanism for resolution of replicative stress at telomeres.
Crosstalk between integrin αvβ3 and ERα contributes to thyroid hormone-induced proliferation of ovarian cancer cells.
A low-salt diet increases the expression of renal sirtuin 1 through activation of the ghrelin receptor in rats.
Downregulation of angiotensin type 1 receptor and nuclear factor-κB by sirtuin 1 contributes to renoprotection in unilateral ureteral obstruction.
Autologous adipose tissue‑derived mesenchymal stem cells are involved in rat liver regeneration following repeat partial hepatectomy.
Repositioning antipsychotic chlorpromazine for treating colorectal cancer by inhibiting sirtuin 1.
Early colonizing Escherichia coli elicits remodeling of rat colonic epithelium shifting toward a new homeostatic state.
IL-20 and IL-20R1 antibodies protect against liver fibrosis.
Combination therapy targeting ectopic ATP synthase and 26S proteasome induces ER stress in breast cancer cells.
Proteome analysis of a hepatocyte-specific BIRC5 (survivin)-knockout mouse model during liver regeneration.
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
ABCG1 is required for pulmonary B-1 B cell and natural antibody homeostasis.
An extract of Rhodobacter sphaeroides reduces cisplatin-induced nephrotoxicity in mice.
Erythrocytosis and pulmonary hypertension in a mouse model of human HIF2A gain of function mutation.
Resveratrol inhibits LPS-induced epithelial-mesenchymal transition in mouse melanoma model.
Synergistic anticancer activity of triptolide combined with cisplatin enhances apoptosis in gastric cancer in vitro and in vivo.
Zhang T, Zhang Z, Shengzhao G, Li X, Liu H, Zhao Y
PLoS genetics 2019 Feb;15(2):e1007925
PLoS genetics 2019 Feb;15(2):e1007925
Preprogramming therapeutic response of PI3K/mTOR dual inhibitor via the regulation of EHMT2 and p27 in pancreatic cancer.
Tian YF, Wang HC, Luo CW, Hung WC, Lin YH, Chen TY, Li CF, Lin CY, Pan MR
American journal of cancer research 2018;8(9):1812-1822
American journal of cancer research 2018;8(9):1812-1822
Neuroprotection against 6-OHDA toxicity in PC12 cells and mice through the Nrf2 pathway by a sesquiterpenoid from Tussilago farfara.
Lee J, Song K, Huh E, Oh MS, Kim YS
Redox biology 2018 Sep;18:6-15
Redox biology 2018 Sep;18:6-15
A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Lactobacillus paracasei CNCM I-3689 reduces vancomycin-resistant Enterococcus persistence and promotes Bacteroidetes resilience in the gut following antibiotic challenge.
Crouzet L, Derrien M, Cherbuy C, Plancade S, Foulon M, Chalin B, van Hylckama Vlieg JET, Grompone G, Rigottier-Gois L, Serror P
Scientific reports 2018 Mar 23;8(1):5098
Scientific reports 2018 Mar 23;8(1):5098
Bisdemethoxycurcumin Suppresses Migration and Invasion of Human Cervical Cancer HeLa Cells via Inhibition of NF-ĸB, MMP-2 and -9 Pathways.
Liao CL, Chu YL, Lin HY, Chen CY, Hsu MJ, Liu KC, Lai KC, Huang AC, Chung JG
Anticancer research 2018 Jul;38(7):3989-3997
Anticancer research 2018 Jul;38(7):3989-3997
EZH2 inhibits autophagic cell death of aortic vascular smooth muscle cells to affect aortic dissection.
Li R, Yi X, Wei X, Huo B, Guo X, Cheng C, Fang ZM, Wang J, Feng X, Zheng P, Su YS, Masau JF, Zhu XH, Jiang DS
Cell death & disease 2018 Feb 7;9(2):180
Cell death & disease 2018 Feb 7;9(2):180
Exosomal MicroRNA-10a Is Associated with Liver Regeneration in Rats through Downregulation of EphA4.
Luo L, Yu ZP, Qin H, Zhu ZX, Liao MH, Liao HT, Yuan KF, Zeng Y
Chinese medical journal 2018 Feb 20;131(4):454-460
Chinese medical journal 2018 Feb 20;131(4):454-460
Overexpression of PCNA Attenuates Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced DNA Damage.
Tsai YC, Wang YH, Liu YC
Journal of nucleic acids 2017;2017:8154646
Journal of nucleic acids 2017;2017:8154646
A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
Ni YL, Hsieh CH, Kim SH, Wang JP, Su CL, Yao CF, Fang K
Apoptosis : an international journal on programmed cell death 2017 Oct;22(10):1235-1245
Apoptosis : an international journal on programmed cell death 2017 Oct;22(10):1235-1245
CD24+ tumor-initiating cells from oral squamous cell carcinoma induce initial angiogenesis in vivo.
Zimmerer RM, Ludwig N, Kampmann A, Bittermann G, Spalthoff S, Jungheim M, Gellrich NC, Tavassol F
Microvascular research 2017 Jul;112:101-108
Microvascular research 2017 Jul;112:101-108
Preventive effect of celecoxib use against cancer progression and occurrence of oral squamous cell carcinoma.
Chiang SL, Velmurugan BK, Chung CM, Lin SH, Wang ZH, Hua CH, Tsai MH, Kuo TM, Yeh KT, Chang PY, Yang YH, Ko YC
Scientific reports 2017 Jul 24;7(1):6235
Scientific reports 2017 Jul 24;7(1):6235
Progression-related loss of stromal Caveolin 1 levels fosters the growth of human PC3 xenografts and mediates radiation resistance.
Panic A, Ketteler J, Reis H, Sak A, Herskind C, Maier P, Rübben H, Jendrossek V, Klein D
Scientific reports 2017 Jan 23;7:41138
Scientific reports 2017 Jan 23;7:41138
Adipose-derived mesenchymal stem cells promote liver regeneration and suppress rejection in small-for-size liver allograft.
Gao W, Zhang L, Zhang Y, Sun C, Chen X, Wang Y
Transplant immunology 2017 Dec;45:1-7
Transplant immunology 2017 Dec;45:1-7
Looping-out mechanism for resolution of replicative stress at telomeres.
Zhang T, Zhang Z, Li F, Hu Q, Liu H, Tang M, Ma W, Huang J, Songyang Z, Rong Y, Zhang S, Chen BP, Zhao Y
EMBO reports 2017 Aug;18(8):1412-1428
EMBO reports 2017 Aug;18(8):1412-1428
Crosstalk between integrin αvβ3 and ERα contributes to thyroid hormone-induced proliferation of ovarian cancer cells.
Hsieh MT, Wang LM, Changou CA, Chin YT, Yang YSH, Lai HY, Lee SY, Yang YN, Whang-Peng J, Liu LF, Lin HY, Mousa SA, Davis PJ
Oncotarget 2017 Apr 11;8(15):24237-24249
Oncotarget 2017 Apr 11;8(15):24237-24249
A low-salt diet increases the expression of renal sirtuin 1 through activation of the ghrelin receptor in rats.
Yang SY, Lin SL, Chen YM, Wu VC, Yang WS, Wu KD
Scientific reports 2016 Sep 7;6:32787
Scientific reports 2016 Sep 7;6:32787
Downregulation of angiotensin type 1 receptor and nuclear factor-κB by sirtuin 1 contributes to renoprotection in unilateral ureteral obstruction.
Yang SY, Lin SL, Chen YM, Wu VC, Yang WS, Wu KD
Scientific reports 2016 Sep 23;6:33705
Scientific reports 2016 Sep 23;6:33705
Autologous adipose tissue‑derived mesenchymal stem cells are involved in rat liver regeneration following repeat partial hepatectomy.
Liu T, Mu H, Shen Z, Song Z, Chen X, Wang Y
Molecular medicine reports 2016 Mar;13(3):2053-9
Molecular medicine reports 2016 Mar;13(3):2053-9
Repositioning antipsychotic chlorpromazine for treating colorectal cancer by inhibiting sirtuin 1.
Lee WY, Lee WT, Cheng CH, Chen KC, Chou CM, Chung CH, Sun MS, Cheng HW, Ho MN, Lin CW
Oncotarget 2015 Sep 29;6(29):27580-95
Oncotarget 2015 Sep 29;6(29):27580-95
Early colonizing Escherichia coli elicits remodeling of rat colonic epithelium shifting toward a new homeostatic state.
Tomas J, Reygner J, Mayeur C, Ducroc R, Bouet S, Bridonneau C, Cavin JB, Thomas M, Langella P, Cherbuy C
The ISME journal 2015 Jan;9(1):46-58
The ISME journal 2015 Jan;9(1):46-58
IL-20 and IL-20R1 antibodies protect against liver fibrosis.
Chiu YS, Wei CC, Lin YJ, Hsu YH, Chang MS
Hepatology (Baltimore, Md.) 2014 Sep;60(3):1003-14
Hepatology (Baltimore, Md.) 2014 Sep;60(3):1003-14
Combination therapy targeting ectopic ATP synthase and 26S proteasome induces ER stress in breast cancer cells.
Chang HY, Huang TC, Chen NN, Huang HC, Juan HF
Cell death & disease 2014 Nov 27;5:e1540
Cell death & disease 2014 Nov 27;5:e1540
Proteome analysis of a hepatocyte-specific BIRC5 (survivin)-knockout mouse model during liver regeneration.
Bracht T, Hagemann S, Loscha M, Megger DA, Padden J, Eisenacher M, Kuhlmann K, Meyer HE, Baba HA, Sitek B
Journal of proteome research 2014 Jun 6;13(6):2771-82
Journal of proteome research 2014 Jun 6;13(6):2771-82
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
May EW, Lin ST, Lin CC, Chang JF, Hung E, Lo YW, Lin LH, Hu RY, Feng CL, Lin DY, Wu SB, Lee WC, Lyu KW, Chou HC, Chan HL
Pharmacological research 2014 Dec;90:1-17
Pharmacological research 2014 Dec;90:1-17
ABCG1 is required for pulmonary B-1 B cell and natural antibody homeostasis.
Baldan A, Gonen A, Choung C, Que X, Marquart TJ, Hernandez I, Bjorkhem I, Ford DA, Witztum JL, Tarling EJ
Journal of immunology (Baltimore, Md. : 1950) 2014 Dec 1;193(11):5637-48
Journal of immunology (Baltimore, Md. : 1950) 2014 Dec 1;193(11):5637-48
An extract of Rhodobacter sphaeroides reduces cisplatin-induced nephrotoxicity in mice.
Chang WW, Liu JJ, Liu CF, Liu WS, Lim YP, Cheng YJ, Lee CH
Toxins 2013 Nov 29;5(12):2353-65
Toxins 2013 Nov 29;5(12):2353-65
Erythrocytosis and pulmonary hypertension in a mouse model of human HIF2A gain of function mutation.
Tan Q, Kerestes H, Percy MJ, Pietrofesa R, Chen L, Khurana TS, Christofidou-Solomidou M, Lappin TR, Lee FS
The Journal of biological chemistry 2013 Jun 14;288(24):17134-44
The Journal of biological chemistry 2013 Jun 14;288(24):17134-44
Resveratrol inhibits LPS-induced epithelial-mesenchymal transition in mouse melanoma model.
Chen MC, Chang WW, Kuan YD, Lin ST, Hsu HC, Lee CH
Innate immunity 2012 Oct;18(5):685-93
Innate immunity 2012 Oct;18(5):685-93
Synergistic anticancer activity of triptolide combined with cisplatin enhances apoptosis in gastric cancer in vitro and in vivo.
Li CJ, Chu CY, Huang LH, Wang MH, Sheu LF, Yeh JI, Hsu HY
Cancer letters 2012 Jun 28;319(2):203-213
Cancer letters 2012 Jun 28;319(2):203-213
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (whole cell lysate) A: HCT116 15 ug B: HCT116 10 ug C: HCT116 5 ug D: HCT116 1 ug 12% SDS PAGE GTX100539 diluted at 1:3000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 12% SDS PAGE GTX100539 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 12% SDS PAGE GTX100539 diluted at 1:3000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE PCNA antibody GTX100539 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 12% SDS PAGE GTX100539 diluted at 1:3000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: PC-12 12% SDS PAGE GTX100539 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCNA antibody detects PCNA protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extract D. 30 ?g HepG2 whole cell lysate/extract12 % SDS-PAGEPCNA antibody (GTX100539) dilution: 1:2500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCNA antibody detects PCNA protein by western blot analysis.A. 30 ?g NIH-3T3 whole cell lysate/extract12% SDS-PAGEPCNA antibody (GTX100539) dilution: 1:2500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCNA antibody detects PCNA protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract12% SDS-PAGEPCNA antibody (GTX100539) dilution: 1:2500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCNA antibody detects PCNA protein by Western blot analysis. Whole cell extracts (30 µg) was separated by 12 % SDS-PAGE, and the membrane was blotted with PCNA antibody (GTX100539) at a dilution of 1:2500.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCNA antibody detects PCNA protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (GTX100539) diluted at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PCNA protein stained by PCNA antibody (GTX100539) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PCNA protein stained by PCNA antibody (GTX100539) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: PCNA protein stained by PCNA antibody (GTX100539) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 £gg of PCBP2 antibody (GTX100539).Western blot analysis was performed using PCBP2 antibody (GTX100539).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCNA antibody detects PCNA protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PCNA antibody (GTX100539) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PCNA antibody (GTX100539) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCNA antibody detects PCNA protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. PCNA antibody (GTX100539) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCNA antibody detects PCNA protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. PCNA antibody (GTX100539) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCNA antibody detects PCNA protein at nucleus in human colon by immunohistochemical analysis. Sample: Paraffin-embedded human colon . PCNA antibody (GTX100539) diluted at 1:500.
