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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- ARG54142 - Provider product page
- Provider
- Arigo
- Product name
- anti-PCNA antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human PCNA protein fragments expressed in E.coli.
- Reactivity
- Human, Mouse, Rat, Hamster, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6B12-D7-H9
- Vial size
- 100 µl
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: PCNA in HeLa, 3T3, COS7, C6 and CHO-K1 cell lysates stained with PCNA antibody [6B12-D7-H9] (ARG54142) (diluted in 1: 1000). Predicted band size: 36KDa. Observed band size: 36KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells stained with PCNA antibody [6B12-D7-H9] (ARG54142) (diluted in 1:100). Fixed in 100% methanol for 2hr at -20¢J.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunoprecipitation: HeLa cell lysates immunoprecipitated and stained by PCNA antibody [6B12-D7-H9] (ARG54142).
