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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [3]
- Immunoprecipitation [3]
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Validation data
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- Product number
- LS-B12937 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ PCNA Antibody (clone 1G7) LS-B12937
- Antibody type
- Monoclonal
- Description
- Purified from ascites by Protein A
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1G7
- Storage
- Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- PCNA monoclonal antibody (M02), clone 1G7 Western Blot analysis of PCNA expression in HeLa.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody (M02), clone 1G7.Lane 1: PCNA transfected lysate(28.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PCNA is approximately 0.1 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PCNA is approximately 0.1 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PCNA is approximately 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of PCNA transfected lysate using anti-PCNA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCNA rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of PCNA transfected lysate using anti-PCNA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCNA rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of PCNA transfected lysate using anti-PCNA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCNA rabbit polyclonal antibody.
