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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- V2218 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PCNA Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific PCNA antibody is suitable for use in Flow cytometry/Immunofluorescence/Western blot/Immunohistochemistry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- PC10
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HeLa cell lysate with PCNA antibody (clone PC10).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HeLa cell lysate with PCNA antibody (clone PC10). Predicted molecular weight ~29kDa, routinely observed at 29~36kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human A431, 293T, HepG2 and MCF7 cell lysate and mouse NIH 3T3 cell lysate with PCNA antibody. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human Raji cell lysate with PCNA antibody. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human human tonsil stained with PCNA antibody (PC10).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human human tonsil stained with PCNA antibody (PC10).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry staining of human A549 cells with PCNA antibody; Red=isotype control, Blue= PCNA antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free PCNA Antibody (clone PC10). Confirmation of Integrity and Purity of the Antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free PCNA antibody (clone PC10) as confirmation of integrity and purity.
