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- Antibody Data
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00005111-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005111-M02, RRID:AB_546874
- Product name
- Mouse Monoclonal PCNA Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PCNA - proliferating cell nuclear antigen
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors.
Mason JM, Logan HL, Budke B, Wu M, Pawlowski M, Weichselbaum RR, Kozikowski AP, Bishop DK, Connell PP
Cancer research 2014 Jul 1;74(13):3546-55
Cancer research 2014 Jul 1;74(13):3546-55
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody (1G7) [H00005111-M02] - Analysis of PCNA transfected lysate using anti-PCNA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCNA MaxPab rabbit polyclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody (1G7) [H00005111-M02] - Analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody (M02), clone 1G7.Lane 1: PCNA transfected lysate(28.8 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody (1G7) [H00005111-M02] - PCNA monoclonal antibody (M02), clone 1G7 Analysis of PCNA expression in Hela.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: PCNA Antibody (1G7) [H00005111-M02] - Detection limit for recombinant GST tagged PCNA is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PCNA Antibody (1G7) [H00005111-M02] - Analysis of monoclonal antibody to PCNA on HeLa cell. Antibody concentration 10 ug/ml.
