12-9910-42

antibody from Invitrogen Antibodies

Targeting: PCNA

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12-9910-42

Provider

Invitrogen Antibodies

Product name

PCNA Monoclonal Antibody (PC10 (3F81)), PE, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The PC10 antibody recognizes the proliferating cell nuclear antigen (PCNA), a 36 kDa protein, also known as polymerase delta auxiliary protein. PC10 antibody reacts with human, mouse, and rat PCNA. The peak expression of PCNA occurs during the S-phase. Applications Reported: The PC10 (a.k.a. 3F81) antibody has been reported for use in intracellular flow cytometric analysis. Applications Tested: This PC10 (a.k.a. 3F81) antibody has been pre-titrated and tested by intracellular flow cytometric analysis of alcohol fixed MOLT-4 cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Yellow dye

Isotype

IgG

Antibody clone number

PC10 (3F81)

Vial size

100 Tests

Concentration

5 µL/Test

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

Inosine pranobex enhances human NK cell cytotoxicity by inducing metabolic activation and NKG2D ligand expression.

McCarthy MT, Lin D, Soga T, Adam J, O'Callaghan CA
European journal of immunology 2020 Jan;50(1):130-137

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Intracellular staining of Molt-4 cells with Anti-Human PCNA FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total cells were used for analysis.

Conjugate

Yellow dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 IP induces dose-dependent cell surface NKG2D ligand expression. (A) NKG2D ligands are not typically expressed on healthy quiescent cells. Stimuli including malignant transformation, viral infection, and proliferative lymphocyte activation are associated with NKG2D ligand induction. Expression can cause cytotoxicity, cytokine secretion, or costimulation through binding to the activating receptor, NKG2D. (B) HEK293T cells were cultured in 5 mM glucose with 0.25, 1, or 2 mM IP for 48 h, and cell surface expression of MICA (2C10) was measured by flow cytometry. A strong dose-dependent increase in MICA expression was observed. Isotype controls (dotted histogram), cells cultured in 5 mM glucose only (light grey shaded histogram) or in 25 mM glucose (dark grey shaded histogram) are also shown. (C) Cells were cultured in 5 or 25 mM glucose with IP in biological triplicates and MICA expression was measured by flow cytometry. In 5 mM glucose, IP produced a significant increase in cell surface MICA expression compared to untreated cells. In 25 mM glucose, a significant increase in MICA expression was observed at higher IP concentrations. (D) HEK293T cells, (E) HT1080 cells (human fibrosarcoma), and (F) HeLa cells (human cervical carcinoma) demonstrate dose-dependent MICA (2C10) expression when cultured with IP. (G) We tested whether IP influenced total cellular MICA levels by staining permeabilized and non-permeabilized cells in parallel. Permeabilized cells displayed the sa

Conjugate

Yellow dye