Explore
Validate
Learn
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 42-9910-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCNA Monoclonal Antibody (PC10 (3F81)), eFluor™ 615, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The PC10 antibody recognizes the proliferating cell nuclear antigen (PCNA), a 36 kDa protein, also known as polymerase delta auxiliary protein. PC10 antibody reacts with human, mouse, and rat PCNA. The peak expression of PCNA occurs during the S-phase. Applications Reported: This PC10 (a.k.a. 3F81) antibody has been reported for use in immunohistochemical and immunocytochemical staining. Applications Tested: This PC10 (a.k.a. 3F81) antibody has been tested by immunocytochemistry of methanol-fixed MDCK cells and can be used at less than or equal to 0.5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- PC10 (3F81)
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references The assessment of proliferating cell nuclear antigen (PCNA)immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S + G2 + M phase fraction (flow cytometric analysis) and prognosis.
Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis.
Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms.
Woods AL, Hall PA, Shepherd NA, Hanby AM, Waseem NH, Lane DP, Levison DA
Histopathology 2002 Sep;41(3A):165-71
Histopathology 2002 Sep;41(3A):165-71
Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis.
Landberg G, Roos G
Cancer research 1991 Sep 1;51(17):4570-4
Cancer research 1991 Sep 1;51(17):4570-4
Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
Waseem NH, Lane DP
Journal of cell science 1990 May;96 ( Pt 1):121-9
Journal of cell science 1990 May;96 ( Pt 1):121-9
Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms.
Hall PA, Levison DA, Woods AL, Yu CC, Kellock DB, Watkins JA, Barnes DM, Gillett CE, Camplejohn R, Dover R
The Journal of pathology 1990 Dec;162(4):285-94
The Journal of pathology 1990 Dec;162(4):285-94
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of methanol-fixed MDCK cells using 0.5 µg/mL anti-Human PCNA (Proliferating Cell Nuclear Antigen) eFluor® 615 (left) and co-localized with DAPI stained nuclei (right).
