Antibody data
- Antibody Data
- Antigen structure
- References [30]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- M00125-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-PCNA Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for PCNA detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat PCNA information: Molecular Weight: 28769 MW; Subcellular Localization: Nucleus . Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- DO-16
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Ethyl 2,2-difluoro-2-(2-oxo-2H-chromen-3-yl) acetate attenuates the malignant biological behaviors of non-small cell lung cancer via suppressing EGFR/PI3K/AKT/mTOR signaling pathway.
Impaired pulp healing associated with underlying disorders in the dental pulp of rats with type 2 diabetes.
Declined circular RNA mitofusin 2 constrains the deterioration of Wilms tumor via modulating microRNA-372-3p/transforming growth factor-β receptor type 2 axis.
Grape Seed Proanthocyanidins Protect Pancreatic β Cells Against Ferroptosis via the Nrf2 Pathway in Type 2 Diabetes.
Overcoming chemoresistance in non-angiogenic colorectal cancer by metformin via inhibiting endothelial apoptosis and vascular immaturity.
The potential mechanism of qinghua quyu jianpi decoction in the treatment of ulcerative colitis based on network pharmacology and experimental validation.
Radiation-induced NF-κB activation is involved in cochlear damage in mice via promotion of a local inflammatory response.
Murine Cytomegalovirus Infection Induced miR-1929-3p Down-Regulation Promotes the Proliferation and Apoptosis of Vascular Smooth Muscle Cells in Mice by Targeting Endothelin A Receptor and Downstream NLRP3 Activation Pathway.
The Protective Effects of γ-Tocotrienol on Muscle Stem Cells Through Inhibiting Reactive Oxidative Stress Production.
Asiaticoside Combined With Carbon Ion Implantation to Improve the Biocompatibility of Silicone Rubber and to Reduce the Risk of Capsule Contracture.
Rapamycin Ameliorates Radiation-Induced Testis Damage in Mice.
Prevotellaceae produces butyrate to alleviate PD-1/PD-L1 inhibitor-related cardiotoxicity via PPARα-CYP4X1 axis in colonic macrophages.
Coordination-Driven Metal-Polyphenolic Nanoparticles toward Effective Anticancer Therapy.
CCDC7 Activates Interleukin-6 and Vascular Endothelial Growth Factor to Promote Proliferation via the JAK-STAT3 Pathway in Cervical Cancer Cells.
Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells.
Biological characteristics of heat shock protein 90 in human liver cancer cells.
Dacomitinib, a new pan-EGFR inhibitor, is effective in attenuating pulmonary vascular remodeling and pulmonary hypertension.
The enhanced antitumor-specific immune response with mannose- and CpG-ODN-coated liposomes delivering TRP2 peptide.
Circadian locomotor output cycles kaput affects the proliferation and migration of breast cancer cells by regulating the expression of E-cadherin via IQ motif containing GTPase activating protein 1.
Comparison of the synergistic effect of lipid nanobubbles and SonoVue microbubbles for high intensity focused ultrasound thermal ablation of tumors.
Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells.
Nanoparticle-enhanced synergistic HIFU ablation and transarterial chemoembolization for efficient cancer therapy.
Pemetrexed induces G1 phase arrest and apoptosis through inhibiting Akt activation in human non small lung cancer cell line A549.
Vinyl chloride monomer (VCM) induces high occurrence of neural tube defects in embryonic mouse brain during neurulation.
Cell proliferation and apoptosis in the fetal and neonatal ovary of guinea pigs.
Lipopolysaccharide inhibits the self-renewal of spermatogonial stem cells in vitro via downregulation of GDNF expression in Sertoli cells.
Comparison of posterior capsule opacification in rabbit eyes receiving different administrations of rapamycin.
FOXA1 promotes tumor cell proliferation through AR involving the Notch pathway in endometrial cancer.
Simvastatin decreases stent-induced in-stent restenosis rate via downregulating the expression of PCNA and upregulating that of p27kip1.
Development-promoting effect of chicken embryo membrane on chicken ovarian cortical pieces of different age.
Zou L, Li X, Lin J, Fang X, Lin J, Zhang L, Zhang Y, Liang Y, Ren J, Liu Y, Huang Z
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Impaired pulp healing associated with underlying disorders in the dental pulp of rats with type 2 diabetes.
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Declined circular RNA mitofusin 2 constrains the deterioration of Wilms tumor via modulating microRNA-372-3p/transforming growth factor-β receptor type 2 axis.
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Grape Seed Proanthocyanidins Protect Pancreatic β Cells Against Ferroptosis via the Nrf2 Pathway in Type 2 Diabetes.
Li H, Zhang H, Wang T, Zhang L, Wang H, Lu H, Yang R, Ding Y
Biological trace element research 2024 Feb 17;
Biological trace element research 2024 Feb 17;
Overcoming chemoresistance in non-angiogenic colorectal cancer by metformin via inhibiting endothelial apoptosis and vascular immaturity.
Li GY, Zhang SJ, Xue D, Feng YQ, Li Y, Huang X, Cui Q, Wang B, Feng J, Bao T, Liu PJ, Lu SY, Wang JC
Journal of pharmaceutical analysis 2023 Mar;13(3):262-275
Journal of pharmaceutical analysis 2023 Mar;13(3):262-275
The potential mechanism of qinghua quyu jianpi decoction in the treatment of ulcerative colitis based on network pharmacology and experimental validation.
Qu F, Li D, Zhang S, Zhang C, Shen A
Journal of ethnopharmacology 2023 Jun 28;310:116396
Journal of ethnopharmacology 2023 Jun 28;310:116396
Radiation-induced NF-κB activation is involved in cochlear damage in mice via promotion of a local inflammatory response.
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Journal of radiation research 2023 Jan 20;64(1):63-72
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Murine Cytomegalovirus Infection Induced miR-1929-3p Down-Regulation Promotes the Proliferation and Apoptosis of Vascular Smooth Muscle Cells in Mice by Targeting Endothelin A Receptor and Downstream NLRP3 Activation Pathway.
He L, Zhou W, Wang L, Tang N, Wang Y, Zhong H, Tang Y, Xi D, He F
Molecular biotechnology 2023 Dec;65(12):1954-1967
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The Protective Effects of γ-Tocotrienol on Muscle Stem Cells Through Inhibiting Reactive Oxidative Stress Production.
Yang S, Yang J, Zhao H, Deng R, Fan H, Zhang J, Yang Z, Zeng H, Kuang B, Shao L
Frontiers in cell and developmental biology 2022;10:820520
Frontiers in cell and developmental biology 2022;10:820520
Asiaticoside Combined With Carbon Ion Implantation to Improve the Biocompatibility of Silicone Rubber and to Reduce the Risk of Capsule Contracture.
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Frontiers in bioengineering and biotechnology 2022;10:810244
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Rapamycin Ameliorates Radiation-Induced Testis Damage in Mice.
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Frontiers in cell and developmental biology 2022;10:783884
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Prevotellaceae produces butyrate to alleviate PD-1/PD-L1 inhibitor-related cardiotoxicity via PPARα-CYP4X1 axis in colonic macrophages.
Chen Y, Liu Y, Wang Y, Chen X, Wang C, Chen X, Yuan X, Liu L, Yang J, Zhou X
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Coordination-Driven Metal-Polyphenolic Nanoparticles toward Effective Anticancer Therapy.
Wu D, Zhou B, Li J, Wang X, Li B, Liang H
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CCDC7 Activates Interleukin-6 and Vascular Endothelial Growth Factor to Promote Proliferation via the JAK-STAT3 Pathway in Cervical Cancer Cells.
Zhou C, He X, Zeng Q, Zhang P, Wang CT
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Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells.
Wang S, Zhang C, Zhang X
Molecular medicine reports 2020 Feb;21(2):589-596
Molecular medicine reports 2020 Feb;21(2):589-596
Biological characteristics of heat shock protein 90 in human liver cancer cells.
Qin L, Huang H, Huang J, Wang G, Huang J, Wu X, Li J, Yi W, Liu L, Huang D
American journal of translational research 2019;11(4):2477-2483
American journal of translational research 2019;11(4):2477-2483
Dacomitinib, a new pan-EGFR inhibitor, is effective in attenuating pulmonary vascular remodeling and pulmonary hypertension.
Yu X, Zhao X, Zhang J, Li Y, Sheng P, Ma C, Zhang L, Hao X, Zheng X, Xing Y, Qiao H, Qu L, Zhu D
European journal of pharmacology 2019 May 5;850:97-108
European journal of pharmacology 2019 May 5;850:97-108
The enhanced antitumor-specific immune response with mannose- and CpG-ODN-coated liposomes delivering TRP2 peptide.
Lai C, Duan S, Ye F, Hou X, Li X, Zhao J, Yu X, Hu Z, Tang Z, Mo F, Yang X, Lu X
Theranostics 2018;8(6):1723-1739
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Circadian locomotor output cycles kaput affects the proliferation and migration of breast cancer cells by regulating the expression of E-cadherin via IQ motif containing GTPase activating protein 1.
Li X, Wang S, Yang S, Ying J, Yu H, Yang C, Liu Y, Wang Y, Cheng S, Xiao J, Guo H, Jiang Z, Wang Z
Oncology letters 2018 May;15(5):7097-7103
Oncology letters 2018 May;15(5):7097-7103
Comparison of the synergistic effect of lipid nanobubbles and SonoVue microbubbles for high intensity focused ultrasound thermal ablation of tumors.
Yao Y, Yang K, Cao Y, Zhou X, Xu J, Liu J, Wang Q, Wang Z, Wang D
PeerJ 2016;4:e1716
PeerJ 2016;4:e1716
Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells.
Niu B, Li B, Wu C, Wu J, Yan Y, Shang R, Bai C, Li G, Hua J
Oncotarget 2016 Nov 22;7(47):77532-77542
Oncotarget 2016 Nov 22;7(47):77532-77542
Nanoparticle-enhanced synergistic HIFU ablation and transarterial chemoembolization for efficient cancer therapy.
You Y, Wang Z, Ran H, Zheng Y, Wang D, Xu J, Wang Z, Chen Y, Li P
Nanoscale 2016 Feb 21;8(7):4324-39
Nanoscale 2016 Feb 21;8(7):4324-39
Pemetrexed induces G1 phase arrest and apoptosis through inhibiting Akt activation in human non small lung cancer cell line A549.
Wu DM, Zhang P, Xu GC, Tong AP, Zhou C, Lang JY, Wang CT
Asian Pacific journal of cancer prevention : APJCP 2015;16(4):1507-13
Asian Pacific journal of cancer prevention : APJCP 2015;16(4):1507-13
Vinyl chloride monomer (VCM) induces high occurrence of neural tube defects in embryonic mouse brain during neurulation.
Quan H, Ma T, Zhao X, Zhao B, Liu Y, Li H
Cellular and molecular neurobiology 2014 May;34(4):619-30
Cellular and molecular neurobiology 2014 May;34(4):619-30
Cell proliferation and apoptosis in the fetal and neonatal ovary of guinea pigs.
Sun SY, Zhang W, Han X, Huang RH, Shi FX
Genetics and molecular research : GMR 2014 Mar 12;13(1):1570-8
Genetics and molecular research : GMR 2014 Mar 12;13(1):1570-8
Lipopolysaccharide inhibits the self-renewal of spermatogonial stem cells in vitro via downregulation of GDNF expression in Sertoli cells.
Zhang X, Shi K, Li Y, Zhang H, Hao J
Reproductive toxicology (Elmsford, N.Y.) 2014 Jun;45:87-93
Reproductive toxicology (Elmsford, N.Y.) 2014 Jun;45:87-93
Comparison of posterior capsule opacification in rabbit eyes receiving different administrations of rapamycin.
Liu H, Zhang Y, Ma H, Zhang C, Fu S
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2014 Jul;252(7):1111-8
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2014 Jul;252(7):1111-8
FOXA1 promotes tumor cell proliferation through AR involving the Notch pathway in endometrial cancer.
Qiu M, Bao W, Wang J, Yang T, He X, Liao Y, Wan X
BMC cancer 2014 Feb 11;14:78
BMC cancer 2014 Feb 11;14:78
Simvastatin decreases stent-induced in-stent restenosis rate via downregulating the expression of PCNA and upregulating that of p27kip1.
Gao C, Xu W, Xiao W, Yu J, Li M
Journal of interventional cardiology 2013 Aug;26(4):384-91
Journal of interventional cardiology 2013 Aug;26(4):384-91
Development-promoting effect of chicken embryo membrane on chicken ovarian cortical pieces of different age.
Yuan JH, Gao JS, Zhan ZF, Liu HW, Jin WJ, Li ZD
Poultry science 2009 Nov;88(11):2415-21
Poultry science 2009 Nov;88(11):2415-21
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon, using PCNA Antibody(M00125-1)PCNA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PCNA Antibody (M00125-1)overnight at 4
- Additional image