Antibody data
- Antibody Data
- Antigen structure
- References [42]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- A00125 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-PCNA Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for PCNA detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Xiao SW, Wang PC, Fu WJ, Wang ZX, Li G, Zhang X
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Pemetrexed induces G1 phase arrest and apoptosis through inhibiting Akt activation in human non small lung cancer cell line A549.
Wu DM, Zhang P, Xu GC, Tong AP, Zhou C, Lang JY, Wang CT
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Asian Pacific journal of cancer prevention : APJCP 2015;16(4):1507-13
Study on mouse model of triple-negative breast cancer: association between higher parity and triple-negative breast cancer.
Huang C, Wang X, Sun B, Li M, Zhao X, Gu Y, Cui Y, Li Y
Targeted oncology 2015 Mar;10(1):85-97
Targeted oncology 2015 Mar;10(1):85-97
Vinyl chloride monomer (VCM) induces high occurrence of neural tube defects in embryonic mouse brain during neurulation.
Quan H, Ma T, Zhao X, Zhao B, Liu Y, Li H
Cellular and molecular neurobiology 2014 May;34(4):619-30
Cellular and molecular neurobiology 2014 May;34(4):619-30
Cell proliferation and apoptosis in the fetal and neonatal ovary of guinea pigs.
Sun SY, Zhang W, Han X, Huang RH, Shi FX
Genetics and molecular research : GMR 2014 Mar 12;13(1):1570-8
Genetics and molecular research : GMR 2014 Mar 12;13(1):1570-8
Comparison of posterior capsule opacification in rabbit eyes receiving different administrations of rapamycin.
Liu H, Zhang Y, Ma H, Zhang C, Fu S
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2014 Jul;252(7):1111-8
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2014 Jul;252(7):1111-8
FOXA1 promotes tumor cell proliferation through AR involving the Notch pathway in endometrial cancer.
Qiu M, Bao W, Wang J, Yang T, He X, Liao Y, Wan X
BMC cancer 2014 Feb 11;14:78
BMC cancer 2014 Feb 11;14:78
Icariin attenuates hypoxia-induced oxidative stress and apoptosis in osteoblasts and preserves their osteogenic differentiation potential in vitro.
Ma HP, Ma XN, Ge BF, Zhen P, Zhou J, Gao YH, Xian CJ, Chen KM
Cell proliferation 2014 Dec;47(6):527-39
Cell proliferation 2014 Dec;47(6):527-39
Simvastatin decreases stent-induced in-stent restenosis rate via downregulating the expression of PCNA and upregulating that of p27kip1.
Gao C, Xu W, Xiao W, Yu J, Li M
Journal of interventional cardiology 2013 Aug;26(4):384-91
Journal of interventional cardiology 2013 Aug;26(4):384-91
Lateral hypothalamic area mediated the aggravated effect of microinjection of Baclofen into cerebellar fastigial nucleus on stress gastric mucosal damage in rats.
Zhu JZ, Fei SJ, Zhang JF, Zhu SP, Liu ZB, Li TT, Qiao X
Neuroscience letters 2012 Feb 16;509(2):125-9
Neuroscience letters 2012 Feb 16;509(2):125-9
Development-promoting effect of chicken embryo membrane on chicken ovarian cortical pieces of different age.
Yuan JH, Gao JS, Zhan ZF, Liu HW, Jin WJ, Li ZD
Poultry science 2009 Nov;88(11):2415-21
Poultry science 2009 Nov;88(11):2415-21
Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma.
Zhang S, Li M, Gu Y, Liu Z, Xu S, Cui Y, Sun B
Journal of experimental & clinical cancer research : CR 2008 Nov 4;27(1):60
Journal of experimental & clinical cancer research : CR 2008 Nov 4;27(1):60
Pancreas duodenal homeobox-1 expression and significance in pancreatic cancer.
Liu T, Gou SM, Wang CY, Wu HS, Xiong JX, Zhou F
World journal of gastroenterology 2007 May 14;13(18):2615-8
World journal of gastroenterology 2007 May 14;13(18):2615-8
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of PCNA using anti-PCNA antibody (A00125). PCNA was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PCNA Antibody (A00125) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image