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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [5]
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- Product number
- NBP1-32075 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32075, RRID:AB_10003898
- Product name
- Rabbit Polyclonal PCNA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Synergistic anticancer activity of triptolide combined with cisplatin enhances apoptosis in gastric cancer in vitro and in vivo.
Li CJ, Chu CY, Huang LH, Wang MH, Sheu LF, Yeh JI, Hsu HY
Cancer letters 2012 Jun 28;319(2):203-213
Cancer letters 2012 Jun 28;319(2):203-213
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody [NBP1-32075] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody diluted at a dilution of 1:2500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug NIH-3T3 whole cell lysate/extract 12 % SDS-PAGE PCNA antibody dilution: 1:2500
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGE PCNA antibody dilution: 1:2500
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PCNA Antibody [NBP1-32075] - Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 ug of PCBP2 antibody. Western blot analysis was performed using PCBP2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Mouse testis. PCNA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human cervical carcinoma. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Rat hind brain. PCNA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human colon. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Mouse liver. PCNA antibody dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
