Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-78105 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-78105, RRID:AB_11026923
- Product name
- Rabbit Polyclonal PCNA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. A BLAST analysis was used to suggest cross reactivity with PCNA protein from human, monkey, dog, mouse, rat, bovine, chicken, fish and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Simian, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.97 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody [NBP1-78105] - Shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts. Approximately 25 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. After blocking, the membrane was incubated with the primary antibody diluted to 1:1000. The membrane was washed and reacted with a 1:10,000 dilution of IRDye(R) 800 conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red).
