Ab155826

antibody from Aladdin Scientific

Targeting: PCNA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab155826

Provider

Aladdin Scientific

Product name

PCNA Mouse mAb

Antibody type

Monoclonal

Description

ApplicationWB: 1/1,000-1/10,000 ;ÊPredicted molecular weight: 29 kDa.Flow: 0.016-2.0_g/mLICC/IF: 0.5_g/mLProtein Function:Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

C04/3D7

Vial size

100_l,10_l,1ml,20_l,50_l

Concentration

Lot by Lot

Storage

Store at +4¡C short term (1-2 weeks). Store at -20¡C or -80¡C. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

PCNA Mouse mAb (Ab155826) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

PCNA Mouse mAb (Ab155826) - Western Blot All lanes: PCNA Antibody (Ab155826) at 0.05 μg/mL Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 29.0 kDa Observed band size: 32.0 kDa Exposure time: 97.0 s 

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

PCNA Mouse mAb (Ab155826) - ICC/IF IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (Ab155826) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow cytometry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

PCNA Mouse mAb (Ab155826) - Flow Cytometry Flow analysis of PCNA (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. PCNA Mouse mAb (Ab155826) at 1/4000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.