Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [11]
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Validation data
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- Product number
- NBP1-89503 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89503, RRID:AB_11010086
- Product name
- Rabbit Polyclonal PCNA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PCNA antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Human glioblastoma multiforme: p53 reactivation by a novel MDM2 inhibitor.
Costa B, Bendinelli S, Gabelloni P, Da Pozzo E, Daniele S, Scatena F, Vanacore R, Campiglia P, Bertamino A, Gomez-Monterrey I, Sorriento D, Del Giudice C, Iaccarino G, Novellino E, Martini C
PloS one 2013;8(8):e72281
PloS one 2013;8(8):e72281
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody [NBP1-89503] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody [NBP1-89503] - Analysis in human cell line MOLT-4.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody [NBP1-89503] - Analysis using Anti-PCNA antibody NBP1-89503 (A) shows similar pattern to independent antibody NBP1-89504 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human skin shows moderate to strong nuclear positivity in epidermal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human small intestine shows strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human liver shows moderate to strong nuclear positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human skeletal muscle shows very weak nuclear positivity in myocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining in human small intestine and skeletal muscle tissues . Corresponding PCNA RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human bone marrow, kidney, lymph node and testis using Anti-PCNA antibody NBP1-89503 (A) shows similar protein distribution across tissues to independent antibody NBP1-89504 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human testis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human small intestine shows strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human bone marrow.