Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP09347PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09347PU-N, RRID:AB_2035832
- Product name
- anti PCNA
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to an internal region of human PCNA protein
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Simian, Xenopus
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.62 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot: Affinity purified anti-PCNA antibody shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts. Approximately 25 µg of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. After blocking, the membrane was incubated with the primary antibody diluted to 1:1000. The membrane was washed and reacted with a 1:10,000 dilution of IRDye(R)800 conjugated affinity purified Goat-anti-Rabbit IgG [H&L] (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red). Other detection systems will yield similar results.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: Formalin-Fixed Paraffin-Embedded (FFPE) breast tissue