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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX101178 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101178, RRID:AB_10617397
- Product name
- Galactosidase alpha antibody [N1C2]
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
Submitted references The lysosomal enzyme alpha-Galactosidase A is deficient in Parkinson's disease brain in association with the pathologic accumulation of alpha-synuclein.
Nelson MP, Boutin M, Tse TE, Lu H, Haley ED, Ouyang X, Zhang J, Auray-Blais C, Shacka JJ
Neurobiology of disease 2018 Feb;110:68-81
Neurobiology of disease 2018 Feb;110:68-81
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa 10% SDS PAGE Galactosidase alpha antibody GTX101178 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Galactosidase alpha antibody detects Galactosidase alpha protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody (GTX101178) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Galactosidase alpha antibody [N1C2] detects Galactosidase alpha protein by western blot analysis. Rat tissue extracts (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody [N1C2] (GTX101178) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of GLA protein from 293T whole cell extracts using 5 £gg of Galactosidase alpha antibody [N1C2] (GTX101178).Western blot analysis was performed using Galactosidase alpha antibody [N1C2] (GTX101178).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Galactosidase alpha(GTX101178) antibody at 1:100 dilution.
