Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX113564 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113564, RRID:AB_11168315
- Product name
- AP2 alpha antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE GTX113564 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AP2 alpha antibody detects AP2 alpha protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 10 % SDS-PAGE, and blotted with AP2 alpha antibody (GTX113564) diluted by 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AP2 alpha antibody detects AP2 alpha protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 10 % SDS-PAGE, and blotted with AP2 alpha antibody (GTX113564) diluted by 1:1000, and developed with Trident femto Western HRP Substrate (GTX14698).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AP2 alpha antibody immunoprecipitates AP2 alpha protein in IP experiments.IP samples: HeLa nuclear extractA. 30 £gg HeLa nuclear extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of AP2 alpha protein by 4 £gg AP2 alpha antibody (GTX113564)10 % SDS-PAGEThe immunoprecipitated AP2 alpha protein was detected by AP2 alpha antibody (GTX113564) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]