Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-52572 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal AP2 alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis using TFAP2A mAb against HEK293 (1) and TFAP2A (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis using TFAP2A mAb against human TFAP2A (AA: 1-100) recombinant protein. (Expected MW is 37 kDa)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis of paraffin-embedded TFAP2A tissues using endometrial cancer mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis of paraffin-embedded ovarian cancer tissues using TFAP2A mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis of MCF-7 cells using TFAP2A mouse mAb (green) and negative control (red).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis of Hela cells using TFAP2A mouse mAb (green) and negative control (red).