- MA1-872 - Invitrogen Antibodies TFAP2A antibody

Submitted references Chaperone-mediated autophagy prevents collapse of the neuronal metastable proteome.

Bourdenx M, Martín-Segura A, Scrivo A, Rodriguez-Navarro JA, Kaushik S, Tasset I, Diaz A, Storm NJ, Xin Q, Juste YR, Stevenson E, Luengo E, Clement CC, Choi SJ, Krogan NJ, Mosharov EV, Santambrogio L, Grueninger F, Collin L, Swaney DL, Sulzer D, Gavathiotis E, Cuervo AM
Cell 2021 May 13;184(10):2696-2714.e25

Directed Differentiation of Human Pluripotent Stem Cells towards Corneal Endothelial-Like Cells under Defined Conditions.

Grönroos P, Ilmarinen T, Skottman H
Cells 2021 Feb 5;10(2)

Aberrant hiPSCs-Derived from Human Keratinocytes Differentiates into 3D Retinal Organoids that Acquire Mature Photoreceptors.

Shrestha R, Wen YT, Ding DC, Tsai RK
Cells 2019 Jan 9;8(1)

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) (Product # MA1-872) shows staining in Hela Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing AP2 (Product # MA1-872) at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) (Product # MA1-872) shows staining in MCF-7 Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing AP2 (Product # MA1-872) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) (Product # MA1-872) shows staining in U251 Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing AP2 (Product # MA1-872) at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of SKP2 was performed using 70% confluent log phase SK-BR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with AP2 alpha Monoclonal Antibody (3B5) (Product MA1-872) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing AP2 (Product # MA1-872) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Comparison of cell cultures with or without BMP inhibition. ( a , e - p ) After 8 days and ( b - d ) 13 days of differentiation, ( a , e ), phase contrast microscope images and ( b - d , f - h ) immunofluorescence images of cell cultures with pathway inhibitor LDN193189 (LDN) show ( b - d ) the AP2alpha-positive polygonal cell colonies where ( f - h ) zona occludens-1 (ZO-1) is localized in tight junctions surrounded by a thick cell mass. ( i - p ) Cell cultures without LDN show that ( i , m ) the thick cell mass is absent, and ( j - l ) AP2alpha is positive for all the cells. ( n - p ) ZO-1 is in tight junctions as well, but the cells are less polygonal. Data conducted with the Regea08/017 hESC line. Scale bars: ( a , f - i , n - p ) 200 um, ( b - d , j - l ) 500 um, ( e , m ) 100 um and ( e , m magnified images) 50 um.

MA1-872

Antibody data