PA5-29769

antibody from Invitrogen Antibodies

Targeting: TFAP2A AP-2, AP2TF, TFAP2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29769

Provider

Invitrogen Antibodies

Product name

AP2 alpha Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.64 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using AP2 alpha Polyclonal Antibody (Product # PA5-29769). Sample (30 µg of whole cell lysate). Lane A: HeLa. 10% SDS PAGE. AP2 alpha Polyclonal Antibody (Product # PA5-29769) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AP2 alpha Polyclonal Antibody detects AP2 alpha protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10 % SDS-PAGE, and blotted with AP2 alpha Polyclonal Antibody (Product # PA5-29769) diluted by 1:1,000, and developed with femto Western HRP Substrate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AP2 alpha Polyclonal Antibody detects AP2 alpha protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10 % SDS-PAGE, and blotted with AP2 alpha Polyclonal Antibody (Product # PA5-29769) diluted by 1:1,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AP2 alpha Polyclonal Antibody immunoprecipitates AP2 alpha protein in IP experiments. IP samples: HeLa nuclear extract. A. 30 µg HeLa nuclear extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of AP2 alpha protein by 4 µg AP2 alpha Polyclonal Antibody (Product # PA5-29769). 10 % SDS-PAGE. The immunoprecipitated AP2 alpha protein was detected by AP2 alpha Polyclonal Antibody (Product # PA5-29769) diluted at 1:500.