Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-29769 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AP2 alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.64 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using AP2 alpha Polyclonal Antibody (Product # PA5-29769). Sample (30 µg of whole cell lysate). Lane A: HeLa. 10% SDS PAGE. AP2 alpha Polyclonal Antibody (Product # PA5-29769) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AP2 alpha Polyclonal Antibody detects AP2 alpha protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10 % SDS-PAGE, and blotted with AP2 alpha Polyclonal Antibody (Product # PA5-29769) diluted by 1:1,000, and developed with femto Western HRP Substrate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AP2 alpha Polyclonal Antibody detects AP2 alpha protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10 % SDS-PAGE, and blotted with AP2 alpha Polyclonal Antibody (Product # PA5-29769) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AP2 alpha Polyclonal Antibody immunoprecipitates AP2 alpha protein in IP experiments. IP samples: HeLa nuclear extract. A. 30 µg HeLa nuclear extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of AP2 alpha protein by 4 µg AP2 alpha Polyclonal Antibody (Product # PA5-29769). 10 % SDS-PAGE. The immunoprecipitated AP2 alpha protein was detected by AP2 alpha Polyclonal Antibody (Product # PA5-29769) diluted at 1:500.