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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- NB600-210 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-210, RRID:AB_10002673
- Product name
- Mouse Monoclonal E2F-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This is specific for human E2F-1.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth.
Zhang L, Zhang S, Yao J, Lowery FJ, Zhang Q, Huang WC, Li P, Li M, Wang X, Zhang C, Wang H, Ellis K, Cheerathodi M, McCarty JH, Palmieri D, Saunus J, Lakhani S, Huang S, Sahin AA, Aldape KD, Steeg PS, Yu D
Nature 2015 Nov 5;527(7576):100-104
Nature 2015 Nov 5;527(7576):100-104
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: E2F-1 Antibody (16G7) [NB600-210] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [16G7] diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: E2F-1 Antibody (16G7) [NB600-210] - Analysis of paraffin-embedded HBL435 xenograft, antibody at 1:200 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: E2F-1 Antibody (16G7) [NB600-210] - Analysis of paraffin-embedded Mouse prostate, antibody at 1:150 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: E2F-1 Antibody (16G7) [NB600-210] - Paraffin-embedded mouse cervix. E2F1 antibody [16G7] diluted at 1:500.
