Antibody data
- Antibody Data
- Antigen structure
- References [37]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- AF2400 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal HNF-3 beta/FoxA2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human HNF-3 beta/FoxA2 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Effects of Natural Progesterone and Synthetic Progestin on Germ Layer Gene Expression in a Human Embryoid Body Model.
Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons.
CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons.
FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation.
Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency.
Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors.
A hPSC-based platform to discover gene-environment interactions that impact human β-cell and dopamine neuron survival.
AAVvector-mediated in vivo reprogramming into pluripotency.
Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines.
CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells.
Triple Staining Including FOXA2 Identifies Stem Cell Lineages Undergoing Hepatic and Biliary Differentiation in Cirrhotic Human Liver.
Cell Expansion During Directed Differentiation of Stem Cells Toward the Hepatic Lineage.
Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS).
Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells.
Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells.
Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency.
Generation of thalamic neurons from mouse embryonic stem cells.
Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells.
A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells.
Physiological characterisation of human iPS-derived dopaminergic neurons.
Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.
Treatment with small molecules is an important milestone towards the induction of pluripotency in neural stem cells derived from human cord blood.
Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2.
A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3.
Complete meiosis from human induced pluripotent stem cells.
CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells.
Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm.
A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells.
Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium.
Linking the p53 tumour suppressor pathway to somatic cell reprogramming.
Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells.
Generation of pluripotent stem cells from adult human testis.
Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure.
Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells.
Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions.
Kim YY, Kim H, Suh CS, Liu HC, Rosenwaks Z, Ku SY
International journal of molecular sciences 2020 Jan 24;21(3)
International journal of molecular sciences 2020 Jan 24;21(3)
Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons.
Riessland M, Kolisnyk B, Kim TW, Cheng J, Ni J, Pearson JA, Park EJ, Dam K, Acehan D, Ramos-Espiritu LS, Wang W, Zhang J, Shim JW, Ciceri G, Brichta L, Studer L, Greengard P
Cell stem cell 2019 Oct 3;25(4):514-530.e8
Cell stem cell 2019 Oct 3;25(4):514-530.e8
CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons.
Calatayud C, Carola G, Fernández-Carasa I, Valtorta M, Jiménez-Delgado S, Díaz M, Soriano-Fradera J, Cappelletti G, García-Sancho J, Raya Á, Consiglio A
Scientific reports 2019 May 2;9(1):6811
Scientific reports 2019 May 2;9(1):6811
FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation.
Lee K, Cho H, Rickert RW, Li QV, Pulecio J, Leslie CS, Huangfu D
Cell reports 2019 Jul 9;28(2):382-393.e7
Cell reports 2019 Jul 9;28(2):382-393.e7
Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency.
Bredenkamp N, Yang J, Clarke J, Stirparo GG, von Meyenn F, Dietmann S, Baker D, Drummond R, Ren Y, Li D, Wu C, Rostovskaya M, Eminli-Meissner S, Smith A, Guo G
Stem cell reports 2019 Dec 10;13(6):1083-1098
Stem cell reports 2019 Dec 10;13(6):1083-1098
Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors.
Ida H, Akiyama T, Ishiguro K, Goparaju SK, Nakatake Y, Chikazawa-Nohtomi N, Sato S, Kimura H, Yokoyama Y, Nagino M, Ko MSH, Ko SBH
Stem cell research & therapy 2018 Oct 25;9(1):277
Stem cell research & therapy 2018 Oct 25;9(1):277
A hPSC-based platform to discover gene-environment interactions that impact human β-cell and dopamine neuron survival.
Zhou T, Kim TW, Chong CN, Tan L, Amin S, Sadat Badieyan Z, Mukherjee S, Ghazizadeh Z, Zeng H, Guo M, Crespo M, Zhang T, Kenyon R, Robinson CL, Apostolou E, Wang H, Xiang JZ, Evans T, Studer L, Chen S
Nature communications 2018 Nov 16;9(1):4815
Nature communications 2018 Nov 16;9(1):4815
AAVvector-mediated in vivo reprogramming into pluripotency.
Senís E, Mosteiro L, Wilkening S, Wiedtke E, Nowrouzi A, Afzal S, Fronza R, Landerer H, Abad M, Niopek D, Schmidt M, Serrano M, Grimm D
Nature communications 2018 Jul 9;9(1):2651
Nature communications 2018 Jul 9;9(1):2651
Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines.
Miquel-Serra L, Duarri A, Muñoz Y, Kuebler B, Aran B, Costa C, Martí M, Comabella M, Malhotra S, Montalban X, Veiga A, Raya A
Stem cell research 2017 Oct;24:155-159
Stem cell research 2017 Oct;24:155-159
CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells.
Wong KG, Ryan SD, Ramnarine K, Rosen SA, Mann SE, Kulick A, De Stanchina E, Müller FJ, Kacmarczyk TJ, Zhang C, Betel D, Tomishima MJ
Stem cell reports 2017 Jul 11;9(1):355-365
Stem cell reports 2017 Jul 11;9(1):355-365
Triple Staining Including FOXA2 Identifies Stem Cell Lineages Undergoing Hepatic and Biliary Differentiation in Cirrhotic Human Liver.
Rogler CE, Bebawee R, Matarlo J, Locker J, Pattamanuch N, Gupta S, Rogler LE
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2017 Jan;65(1):33-46
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2017 Jan;65(1):33-46
Cell Expansion During Directed Differentiation of Stem Cells Toward the Hepatic Lineage.
Raju R, Chau D, Cho DS, Park Y, Verfaillie CM, Hu WS
Stem cells and development 2017 Feb 15;26(4):274-284
Stem cells and development 2017 Feb 15;26(4):274-284
Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS).
Kuebler B, Aran B, Miquel-Serra L, Muñoz Y, Ars E, Bullich G, Furlano M, Torra R, Marti M, Veiga A, Raya A
Stem cell research 2017 Dec;25:291-295
Stem cell research 2017 Dec;25:291-295
Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells.
Kondo Y, Toyoda T, Ito R, Funato M, Hosokawa Y, Matsui S, Sudo T, Nakamura M, Okada C, Zhuang X, Watanabe A, Ohta A, Inagaki N, Osafune K
Diabetologia 2017 Aug;60(8):1454-1466
Diabetologia 2017 Aug;60(8):1454-1466
Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells.
Sampaziotis F, de Brito MC, Geti I, Bertero A, Hannan NR, Vallier L
Nature protocols 2017 Apr;12(4):814-827
Nature protocols 2017 Apr;12(4):814-827
Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency.
Lee JH, Laronde S, Collins TJ, Shapovalova Z, Tanasijevic B, McNicol JD, Fiebig-Comyn A, Benoit YD, Lee JB, Mitchell RR, Bhatia M
Cell reports 2017 Apr 4;19(1):20-35
Cell reports 2017 Apr 4;19(1):20-35
Generation of thalamic neurons from mouse embryonic stem cells.
Shiraishi A, Muguruma K, Sasai Y
Development (Cambridge, England) 2017 Apr 1;144(7):1211-1220
Development (Cambridge, England) 2017 Apr 1;144(7):1211-1220
Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells.
Sato H, Idiris A, Miwa T, Kumagai H
Scientific reports 2016 Aug 10;6:31063
Scientific reports 2016 Aug 10;6:31063
A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells.
Tsankov AM, Akopian V, Pop R, Chetty S, Gifford CA, Daheron L, Tsankova NM, Meissner A
Nature biotechnology 2015 Nov;33(11):1182-92
Nature biotechnology 2015 Nov;33(11):1182-92
Physiological characterisation of human iPS-derived dopaminergic neurons.
Hartfield EM, Yamasaki-Mann M, Ribeiro Fernandes HJ, Vowles J, James WS, Cowley SA, Wade-Martins R
PloS one 2014;9(2):e87388
PloS one 2014;9(2):e87388
Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.
Villa-Diaz LG, Kim JK, Lahann J, Krebsbach PH
Stem cells translational medicine 2014 Dec;3(12):1410-7
Stem cells translational medicine 2014 Dec;3(12):1410-7
Treatment with small molecules is an important milestone towards the induction of pluripotency in neural stem cells derived from human cord blood.
Szablowska-Gadomska I, Sypecka J, Zayat V, Podobinska M, Pastwinska A, Pienkowska-Grela B, Buzanska L
Acta neurobiologiae experimentalis 2012;72(4):337-50
Acta neurobiologiae experimentalis 2012;72(4):337-50
Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
Prabakar KR, Domínguez-Bendala J, Molano RD, Pileggi A, Villate S, Ricordi C, Inverardi L
Cell transplantation 2012;21(6):1321-39
Cell transplantation 2012;21(6):1321-39
Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2.
Montserrat N, Ramírez-Bajo MJ, Xia Y, Sancho-Martinez I, Moya-Rull D, Miquel-Serra L, Yang S, Nivet E, Cortina C, González F, Izpisua Belmonte JC, Campistol JM
The Journal of biological chemistry 2012 Jul 13;287(29):24131-8
The Journal of biological chemistry 2012 Jul 13;287(29):24131-8
A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3.
Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ
Journal of cell science 2011 Jun 15;124(Pt 12):1992-2000
Journal of cell science 2011 Jun 15;124(Pt 12):1992-2000
Complete meiosis from human induced pluripotent stem cells.
Eguizabal C, Montserrat N, Vassena R, Barragan M, Garreta E, Garcia-Quevedo L, Vidal F, Giorgetti A, Veiga A, Izpisua Belmonte JC
Stem cells (Dayton, Ohio) 2011 Aug;29(8):1186-95
Stem cells (Dayton, Ohio) 2011 Aug;29(8):1186-95
CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells.
Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H
Stem cells (Dayton, Ohio) 2011 Apr;29(4):609-17
Stem cells (Dayton, Ohio) 2011 Apr;29(4):609-17
Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm.
Chayosumrit M, Tuch B, Sidhu K
Biomaterials 2010 Jan;31(3):505-14
Biomaterials 2010 Jan;31(3):505-14
A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
Klim JR, Li L, Wrighton PJ, Piekarczyk MS, Kiessling LL
Nature methods 2010 Dec;7(12):989-94
Nature methods 2010 Dec;7(12):989-94
Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells.
Raya A, Rodríguez-Pizà I, Guenechea G, Vassena R, Navarro S, Barrero MJ, Consiglio A, Castellà M, Río P, Sleep E, González F, Tiscornia G, Garreta E, Aasen T, Veiga A, Verma IM, Surrallés J, Bueren J, Izpisúa Belmonte JC
Nature 2009 Jul 2;460(7251):53-9
Nature 2009 Jul 2;460(7251):53-9
Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium.
Nakanishi M, Kurisaki A, Hayashi Y, Warashina M, Ishiura S, Kusuda-Furue M, Asashima M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Jan;23(1):114-22
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Jan;23(1):114-22
Linking the p53 tumour suppressor pathway to somatic cell reprogramming.
Kawamura T, Suzuki J, Wang YV, Menendez S, Morera LB, Raya A, Wahl GM, Izpisúa Belmonte JC
Nature 2009 Aug 27;460(7259):1140-4
Nature 2009 Aug 27;460(7259):1140-4
Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells.
Zhang D, Jiang W, Liu M, Sui X, Yin X, Chen S, Shi Y, Deng H
Cell research 2009 Apr;19(4):429-38
Cell research 2009 Apr;19(4):429-38
Generation of pluripotent stem cells from adult human testis.
Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Bühring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
Nature 2008 Nov 20;456(7220):344-9
Nature 2008 Nov 20;456(7220):344-9
Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure.
Cho CH, Parashurama N, Park EY, Suganuma K, Nahmias Y, Park J, Tilles AW, Berthiaume F, Yarmush ML
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Mar;22(3):898-909
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Mar;22(3):898-909
Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells.
Zhang P, Li J, Tan Z, Wang C, Liu T, Chen L, Yong J, Jiang W, Sun X, Du L, Ding M, Deng H
Blood 2008 Feb 15;111(4):1933-41
Blood 2008 Feb 15;111(4):1933-41
Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions.
Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S
Proceedings of the National Academy of Sciences of the United States of America 2006 May 2;103(18):6907-12
Proceedings of the National Academy of Sciences of the United States of America 2006 May 2;103(18):6907-12
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human HNF‑3ß/FoxA2 by Western Blot. Detection of Human HNF‑3ß/FoxA2 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- HNF-3ß/FoxA2 in Human Liver. HNF-3ß/FoxA2 in Human Liver.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of HNF-3ß/FoxA2-regulated Genes by Chromatin Immunoprecipitation. Detection of HNF-3ß/FoxA2-regulated Genes by Chromatin Immunoprecipitation.