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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324382 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal FOXA2 Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal FOXA2 Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- FOXA2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- FOXA2 Antibody (Center) (Cat. #TA324382) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FOXA2 antibody detected the FOXA2 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of A549 cell stained with FOXA2 Antibody (Center)(Cat#TA324382).A549 cells were incubated with FOXA2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . FOXA2 immunoreactivity is localized to Nucleus significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- FOXA2 Antibody (Center) (Cat. #TA324382)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXA2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
