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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA500073 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500073, RRID:AB_2104997
- Product name
- Anti-FOXA2 mouse monoclonal antibody, clone OTI3C10 (formerly 3C10)
- Antibody type
- Monoclonal
- Description
- Anti-FOXA2 mouse monoclonal antibody, clone OTI3C10 (formerly 3C10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FOXA2
- Isotype
- IgG
- Antibody clone number
- OTI3C10
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA2(RC211408) (RC211408, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 1 cell line by using anti-FOXA2 monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-FOXA2 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-FOXA2 mouse monoclonal antibody (TA500073) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY FOXA2(RC211408)
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-FOXA2 mouse monoclonal antibody (TA500073) at 1:50 dilution.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-FOXA2 (TA500073) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-FOXA2 (TA500073) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY FOXA2(RC211408)(Red) or empty vector control plasmid(Blue) were immunostained with anti-FOXA2 mouse monoclonal(TA500073, Dilution 1:1,000), and then analyzed by flow cytometry.
- Validation comment
- FC
