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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27158 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2. Predicted reactivity: Mouse (100%), Rat (100%), Japanese Medaka (100%), Chicken (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FOXA2 Polyclonal Antibody (Product # PA5-27158). Sample (30 µg of whole cell lysate). Lane A: Hep G2. 10% SDS PAGE. HNF-3-beta antibody . FOXA2 Polyclonal Antibody (Product # PA5-27158) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of FOXA2 was performed in frozen sectioned mouse E105 embryonic neuron tube tissue using FOXA2 Polyclonal Antibody (Product # PA5-27158) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
